We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to Creating and screening for SNPs
  • SNP detection with knockin screening kit
  • Oligo design tool for SNP screening
  • Sign up: SNP engineering webinar
  • Guide-it SNP Screening Kit FAQs
SNP screening kit product page Guide-it SNP Screening Kit product page
Guide-it SNP Screening Kit oligo design tool SNP screening kit oligo design tool
Webinars SNP detection webinar
Home › Learning centers › Gene function › Gene editing › Creating and screening for SNPs › Guide-it SNP Screening Kit FAQs

Gene editing

  • Gene editing product finder
  • Gene editing tools and information
    • sgRNA design tools
    • Tools for successful CRISPR/Cas9 genome editing
    • Gene editing posters
    • Customer data for Guide-it products
    • How to design sgRNA sequences
    • Introduction to the CRISPR/Cas9 system
    • Gene editing of CD3+ T cells and CD34+ HSCs
  • CRISPR/Cas9 knockouts
    • Mutation detection kit comparison
    • Screening for effective guide RNAs
    • Monoallelic versus biallelic mutants
    • Indel identification kit for mutation characterization
  • CRISPR/Cas9 knockins
    • Choosing an HDR template format
    • Homology-directed repair FAQs
    • Mouse CRISPR knockin protocol
    • Site-specific gene knockins using long ssDNA
    • Efficient CRISPR/Cas9-mediated knockins in iPS cells
    • Oligo design tool for detecting precise insertions
      • Oligo design tool user guide (insertions)
  • Genome-wide screening
    • CRISPR library screening
    • CRISPR library screening webinar
    • Phenotypic screen using sgRNA library system
  • Creating and screening for SNPs
    • SNP detection with knockin screening kit
    • Oligo design tool for SNP screening
      • Oligo design tool user guide (SNPs)
    • Sign up: SNP engineering webinar
    • Guide-it SNP Screening Kit FAQs
  • CRISPR/Cas9 delivery methods
    • Electroporation-grade Cas9 for editing in diverse cell types
    • CRISPR/Cas9 gene editing with AAV
    • CRISPR/Cas9 gesicles overview
    • Cas9 Gesicles—reduced off-target effects
    • sgRNA-Cas9 delivery to many cell types
    • Tet-inducible Cas9 for gene editing
  • Cre recombinase
    • Control your Cre recombinase experiments
    • Fast Cre delivery with gesicle technology
New products
Need help?
Contact Sales
SNP screening kit product page Guide-it SNP Screening Kit product page
Guide-it SNP Screening Kit oligo design tool SNP screening kit oligo design tool
Webinars SNP detection webinar

Guide-it SNP Screening Kit FAQs

The Guide-it SNP Screening Kit provides a sensitive and accurate method for high-throughput detection of single-nucleotide substitutions in clonal populations using a simple enzymatic assay.

The principle of the assay is described in the following video:

What is the kit workflow?

The assay employed by the Guide-it SNP Screening Kit consists of the following steps:

Guide-it SNP Screening Kit workflow

As indicated, this workflow enables analysis of 96 samples in less than 4 hours.

What oligos do I need to order to perform the assay?

Assaying a given nucleotide substitution with the Guide-it SNP Screening Kit requires the following oligos:

  • Forward and reverse primers (orange) for PCR amplification of the genomic region containing the target site

Forward and reverse primers (orange) for PCR amplification of the genomic region containing the target site

  • Displacement oligo (green) and flap-probe oligo (purple) for detecting the edited nucleotide in the PCR product, and SNP and wild-type (WT) control oligos (blue) for verifying the performance of the designed displacement and flap-probe oligos

Displacement oligo (green) and flap-probe oligo (purple) for detecting the edited nucleotide in the PCR product, and SNP and wild-type (WT) control oligos (blue) for verifying the performance of the designed displacement and flap-probe oligos

Oligos used with the Guide-it SNP Screening Kit do not require special modifications such as fluorescent labels or quenchers, but the kit protocol does require the inclusion of a hexanediol (/3C6/) blocking modification on the 3' end of the flap-probe oligo. Inclusion of this modification is a standard option provided with oligo synthesis services, and no special purification step is needed in this case, only standard desalting. To simplify the oligo design process, we have developed an online software tool that outputs assay-specific oligo sequences in a ready-to-order format. Additional information regarding the design of the assay-specific oligos is provided in the Guide-it SNP Screening Kit User Manual.

How does Guide-it Flapase detect the edited nucleotide?

Guide-it Flapase is a recombinant, structure-specific nuclease from Takara Bio; it recognizes and cleaves double-flap structures, but is unable to cleave gapped structures. The Guide-it SNP Screening Kit employs this feature to assay for single-nucleotide substitutions: the main difference between the double-flap and the gapped structures is whether or not hybridization occurs between the flap-probe oligo and the PCR product at the position of the interrogated base.

Tripartite structures formed by hybridization of the displacement oligo, flap-probe oligo, and PCR product during the SNP screening assay

Tripartite structures formed by hybridization of the displacement oligo, flap-probe oligo, and PCR product during the SNP screening assay. Scheme showing the different tripartite structures generated in an assay designed to detect a C→T substitution. Annealing of the displacement oligo (green) and flap-probe oligo (purple, encoding for the edited nucleotide, T) to the PCR product (blue) forms either of two structures depending on whether the interrogated base in the PCR product is edited or wild-type. Double-flap structure (left). In a scenario where the interrogated base is edited, the flap-probe oligo containing the complementary nucleotide forms a complete base pairing at the target site. Gapped structure (right). If the editing event is unsuccessful and the interrogated base is wild-type, there isn't a complete base pairing at the target site, and a gap is formed.

What is included in the kit?

The Guide-it SNP Screening Kit includes all necessary reagents for extraction and amplification of genomic DNA from mammalian cell samples, and for performing the Guide-it Flapase enzymatic assay:

Guide-it SNP Screening Kit632652 (100 rxns)632653 (400 rxns)
MightyPrep Reagent for DNA (Store at 4°C) 9182A 9182
MightyPrep Reagent for DNA 5 ml 20 ml
Guide-it SNP Control Set (Store at –20°C) 632654 632654
Guide-it SNP Positive Control Mix 300 µl 300 µl
Guide-it SNP Negative Control Mix 300 µl 300 µl
Guide-it Flap Reagents (Store at –20°C) 632655 (100 rxns) 632656 (400 rxns)
Terra PCR Direct Polymerase Mix 50 µl 200 µl
2X Terra PCR Direct Buffer (with Mg2+, dNTP) 750 µl x 2 1 ml x 5
Dilution Buffer 8 ml 32 ml
RNase-free Water 10 ml 40 ml
Annealing Buffer 350 µl 1.4 ml
Flapase Buffer 350 µl 1.4 ml
Guide-it Flapase 100 µl 400 µl
Guide-it Flap Detector (40X) 50 µl 200 µl

In addition to user-provided oligos specific for each assay, performing the assay requires a thermal cycler and fluorescence plate reader.

What do I need for the detection of the assay signal?

Detection of the assay signal requires only a standard fluorescence plate reader that enables excitation at 485 nm and capture at 535 nm. If the plate reader to be used has different possible filter sets, the Guide-it SNP Control Set provided in the kit can be used to determine the optimal combination of filters.

Can a qPCR thermal cycler be used for fluorescence detection?

Yes, a qPCR thermal cycler can be used. The Guide-it SNP Screening Kit employs an end-point assay that doesn't require real-time monitoring of the flapase reaction, but a qPCR machine can be used during the enzymatic assay to periodically measure fluorescence (e.g., every 3 minutes) to follow the kinetics of the reaction and upon completion of the reaction.

Does the Guide-it SNP Screening Kit allow for detection of any nucleotide substitution regardless of the genomic target site?

Yes. The Guide-it SNP Screening Kit has been used successfully to analyze SNPs at 18 different loci within the human genome (corresponding to 14 different genes, see table); a subset of the results are shown in the graph below.

Detecting different nucleotide substitutions from genomic DNA

Detecting different nucleotide substitutions from genomic DNA. Genomic DNA samples (obtained from the Coriell Institute) which were either wild-type or homozygous for the indicated substitutions were analyzed using the Guide-it SNP Screening Kit. All substitutions were successfully detected, as demonstrated by the strong fluorescent signals obtained for samples that were homozygous (+/+, blue) for the indicated substitutions relative to signals obtained for wild-type (–/–, orange) and negative control (NT, gray) samples.

All the genomic loci and the nucleotide substitutions we have tested so far are listed in the table below:

GeneNucleotide substitutionPosition
F2 G→A g.20210
A→G
HFE C→G c.187C→G (p.His63Asp)
G→C c.187G→C
ITPA C→A c.94C→A  (p.Pro32Thr)
A→C c.94A→C
MAN2B1 A→T c.212A→T (p.His71Leu)
T→A c.212T→A
DBT(E2) G→T c.487G→T(p.Glu163Ter)
T→G c.487T→G
GALT G→C c.292G→C (p.Asp98His)
C→G c.292C→G
MTHFR A→C c.1289A→C (p.Glu429Ala)
C→A c.1289
SMPD1 T→C c.905T→C (p.Leu302Pro)
C→T c.905C→T
NCP1 C→T c.2793C→T(p.Asn931Asn)
T→C c.2793T→C
A→G c.2572A→G(p.Ile858Val)
G→A c.2572G→A
CFTR T→A c.3434T→A (p. Met1101Lys)
A→T c.3434A→T
REQCL4 C→T g.1488C→T (p.Ser246Ser)
G→C g.1551G→C (p.Glu267Asp)
G→A g.5321G→A (p.Arg1005Gln)
DPM1 C→G c.274C→G (p.Arg92Gly)
TUBGCP6 G→T c.5458G→T (p.Gly1820Ter)
T→G c.5458T→G
FAH G→A c.786G→A (p.Trp262Ter)
G→A c.1009G→A (p.Gly337Ser)

Can I use the kit if I have no idea what the substitution is?

Each assay performed with the Guide-it SNP Screening Kit is designed to detect a specific single-nucleotide substitution at a particular genomic location. The sequence of the flap-probe oligo determines which nucleotide will be detected at a chosen target site. To be able to identify any nucleotide at a given site, one must perform four independent assays, with each assay using a different flap-probe oligo designed to specifically detect a G, C, A, or T at the target site (i.e., one specific flap-probe oligo per possible nucleotide).

Can the Guide-it SNP Screening Kit be used for genotyping?

Yes, you will need to design one specific flap-probe per possible nucleotide at the target site and perform independent assays.

Using the Guide-it SNP Screening Kit for genotyping

Using the Guide-it SNP Screening Kit for genotyping. Samples from the Coriell Institute carrying SNPs at either of two genomic loci (NCP1 or CFTR genes) were analyzed using the Guide-it SNP Screening Kit (graphs, top) and by Sanger sequencing (chromatograms, bottom). Panel A. Analysis of NCP1. The analysis determined which samples were homozygous or heterozygous for an A→G substitution by employing two flap-probe oligos in independent assays designed to detect either A or G. Panel B. Analysis of CFTR. Analysis of samples that were either wild-type or homozygous for the indicated T→A substitution. All results for both panels were confirmed by Sanger sequencing (displayed below the graphs).

Can the kit be used to detect successful homologous recombination (HR) in edited populations?

Although the Guide-it SNP Screening Kit was specifically intended for detection of SNPs in clonal cell lines, it can be used to detect successful HR in an edited population with a detection limit around 5%*. Download our poster to see data relating to this application. Visit our technical support page if you need more information about how to use the kit for the detection of HR.

*For this particular application, we recommend that genomic DNA should be extracted from the edited population using NucleoSpin Tissue Columns (Cat. #740952.250S).

Detection of a CRISPR/Cas9-generated nucleotide substitution in a pool of edited cells using RFLP analysis and the Guide-it SNP Screening Kit

Detection of a CRISPR/Cas9-generated nucleotide substitution in a pool of edited cells using RFLP analysis and the Guide-it SNP Screening Kit. Creation of an isogenic hiPSC line carrying a SNP related to tyrosinemia (c.1009G→A) in the FAH gene using CRISPR/Cas9 technology. Panel A. Design of sgRNAs and HR donor template. Different sgRNAs (indicated by #1, #2, and #3) located around the target site (in bold) were tested. A synthetic short oligo encoding for the SNP (in red) was used as a donor template. The edited sequence encodes for a new PvuII restriction site. Panel B. Results of RFLP analysis. Analysis of the cells electroporated with Cas9-sgRNA complexes (RNPs) without (1) or with (2) the donor template. The results suggest that only sgRNA #3 triggered the desired G→A substitution due to the appearance of the extra band in Lane 2 (indicated by blue arrow). Panel C. Results of analysis using the Guide-it SNP Screening Kit. After extraction of genomic DNA using NucleoSpin Tissue Columns, The Guide-it SNP Screening Kit was used to determine which experimental conditions were successful in introducing the SNP. Consistent with the RFLP results, presence of the G→A substitution was only detected in cells edited using the RNP with sgRNA #3 in combination with the HR donor template. By analysis of more than 100 clones it was determined that the percentage of HR was approximately 24%. Panel D. PCR products obtained from the edited cell population using sgRNA #3 were serially diluted with wild-type PCR products, such that the G→A substitution was represented at the frequencies indicated in the graph. The samples were then analyzed using the Guide-it SNP Screening Kit in order to determine the detection limit (gray line).

Do I need to use column-purified genomic DNA?

No. The cells are lysed with MightyPrep Reagent for DNA, and the supernatant from the lysate is used directly as template for the PCR to amplify the genomic target sequence without the need for extra purification steps.

Which cell types have been analyzed using the kit? Can the kit be used for suspension cells?

The Guide-it SNP Screening Kit has been used successfully to analyze a variety of cell types, including cells grown in suspension (Jurkat cells) and adherent cells (fibroblasts, hiPSCs).

Analysis of Jurkat cells using the Guide-it SNP Screening Kit.

Analysis of Jurkat cells using the Guide-it SNP Screening Kit. Jurkat cells were plated at different cell densities in a 96-well plate. After 48 hours, genomic DNA was extracted using MightyPrep Reagent for DNA and the Guide-it SNP Screening Kit was successfully applied to detect the indicated A→G substitution.

What cell densities are suitable for analysis with the kit?

Clonal cell lines in a 96-well plate can have different growth rates, and therefore the protocol has been optimized for a wide range of cell densities (from 2 x 104 to 2 x 105 cells per well).

However, if this assay is being performed for the detection of homologous recombination we recommend that the assay be performed with genomic DNA that has been purified using NucleoSpin Tissue Columns (Cat. # 740952.250S).

Assessing the performance of the Guide-it SNP Screening Kit across a range of cell densities

Assessing the performance of the Guide-it SNP Screening Kit across a range of cell densities. Panel A. The Guide-it SNP Screening Kit was used to assay for a C→G substitution in the DPM1 gene. Two cell lines (one wild-type, the other carrying the SNP) were plated at different densities in a 96-well plate. After 48 hours, they were analyzed using the Guide-it SNP Screening Kit. Fluorescent signals (arbitrary units) and signal-to-noise ratios were largely comparable over a range of 1.8 x 103–1.2 x 105 cells. Panel B. Microscopy images of the primary fibroblasts that were seeded at different cell densities from 2 x 105 (left) to 2 x 104 (right) cells per well.

Can the protocol for the Guide-it SNP Screening Kit be adapted for use with 384-well plates?

We have not tested the kit protocol using 384-well plates; however, theorizing such an adaptation, the only modification we can foresee at this time is a reduction in reaction volumes. Please note that some troubleshooting may be required to ensure successful setup of the PCR reaction and enzymatic assay and to confirm that the resulting fluorescent signal is strong enough to be detected using your chosen method. If you need more assistance with this, please contact our Technical Support team.

Can the kit assay be used to distinguish homozygotes from heterozygotes?

As demonstrated by the data shown below, each assay performed with the kit provides a qualitative output rather than a quantitative measure of a substitution's frequency. Therefore, a single Guide-it SNP screening assay using the current single-color chemistry cannot be used to distinguish homozygous clones from heterozygous clones (see FAQ above, "Could the Guide-it SNP Screening Kit be used for genotyping?" for information about how the kit can be used to assess zygosity). We are developing a new version of the kit that will enable simultaneous detection of either of two bases at the same position (e.g., SNP and wild type) using two Guide-it flap detectors in tandem (one triggering a red fluorescent signal and the other one producing a green signal).

Comparison of assay results obtained for homozygous, heterozygous, and wild-type cell samples

Comparison of assay results obtained for homozygous, heterozygous, and wild-type cell samples. The Guide-it SNP Screening Kit was used to assay for each indicated substitution in samples that were homozygous (+/+, blue), heterozygous (+/–, purple), or wild-type (–/–, orange). For each case, fluorescent signals obtained for homozygous and heterozygous samples (blue bars and purple bars, respectively) were of comparable value.

How clean does the PCR of the genomic target sequence need to be?

The assay performs well regardless of whether extra PCR products are generated during amplification of the genomic target sequence. See the image below for example PCR amplification results that have been successfully used to detect nucleotide substitutions with this kit.

Examples of PCR-amplified target DNAs used as samples with the Guide-it SNP Screening Kit

Examples of PCR-amplified target DNAs used as samples with the Guide-it SNP Screening Kit. PCR products visible in each gel lane were successfully analyzed for nucleotide substitutions involving the indicated genomic loci using the Guide-it SNP Screening Kit. As indicated by the gel, the size of the amplified target can range between 200 bp and 700 bp. For SNP detection assays involving the DBT(E2) and MTHFR gene sequences, amplicons of two different sizes were successfully used.

How sensitive is the Guide-it SNP Screening Kit to the presence of mutations close to the site of the nucleotide substitution being assayed?

The Guide-it SNP Screening Kit is highly sensitive to the presence of mutations in the genomic target sequence surrounding the site of the substitution being assayed. The displacement oligo and the flap-probe oligo anneal to the region immediately adjacent to the SNP, and the presence of unknown mutations in that region would affect the annealing of the oligos with the PCR product and the formation of the double-flap structure.

Sensitivity of the Guide-it SNP Screening Kit to mutations involving the genomic target sequence

Sensitivity of the Guide-it SNP Screening Kit to mutations involving the genomic target sequence. The c.786G→A substitution in the FAH gene was engineered in hiPSCs by RNP electroporation together with either of two HR templates; one HR template (#1) encoded a sequence which included only the G→A substitution (in red), another template (#2) included an additional G→T substitution (in green) which generated a new SmlI restriction site (allowing detection of the substitution via RFLP). Two different displacement oligos were designed for both scenarios (i.e., G→A substitution vs. G→A and G→T substitutions in tandem), and used to assay samples obtained from the various edited cell populations. The oligo designed to assay the G→A substitution (displacement #1) only generated a strong signal for the sample electroporated with template #1. The complementary result was true for the displacement oligo designed to assay the two substitutions. These results show the sensitivity of the assay towards the sequence surrounding the edited base.

Are there any limitations or special considerations regarding what target sequences can be analyzed with the kit?

The only design limitation one must consider when designing the probes is that the displacement oligo must not encode the sequences 5'-GGAGn-3' or 5'-GGAGNn-3' at its 3' terminus (where N can be any base, and n is the extra noncomplementary base that depends on the substitution being assayed). Displacement oligos containing these sequences will generate a background signal in the enzymatic assay. In these scenarios it is advisable to redesign the assay to target the opposite strand of the PCR product, such that the displacement oligo doesn't encode the restricted sequence at its 3' terminus (see example in figure below).

Example of a limitation in the design of the displacement oligo

Example of a limitation in the design of the displacement oligo. In this scenario, the displacement oligo designed to hybridize with the PCR product (in the 3'→5' orientation) encodes the restricted sequence at its 3' terminus (Panel A). A viable alternative (Panel B) targets the opposite strand of the PCR product (in the 5'→3' orientation), avoiding the inclusion of the restricted sequence at the 3' terminus of the displacement oligo.

Can the kit only be used to detect single-nucleotide substitutions? Would it be suitable for detecting longer insertions?

The current version of the Guide-it SNP Screening Kit can only be used to detect single-nucleotide substitutions, not longer insertions.

We are, however, in the process of developing a new version of the kit that will enable detection of longer insertions using two Guide-it Flap Detectors in tandem. One detector yields a red fluorescent signal while the other one generates a green signal, allowing simultaneous detection of seamless insertions at both the 5' and 3' ends of the recombinant sequence. We have applied this updated approach for detecting the fusion of a myc tag to an endogenous gene in hiPSCs (a poster including this data is available for download).

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2023 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Diagnostic solutions
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Services & Support
  • OEM
  • Instrument services
  • Stem cell services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • cDNA synthesis
  • Real-time PCR
  • Nucleic acid purification
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • Vaccine development
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us