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DNA extraction for direct PCR: MightyPrep Reagent for DNA

Easily prepare DNA extracts from animal tissues (such as mouse tail), plant tissues, blood, processed food, and soil for PCR applications

MightyPrep Reagent for DNA is designed to easily prepare DNA extracts from animal tissues (such as mouse tail), plant tissues, blood, processed food, and soil that can be used directly as PCR templates. DNA extracts are prepared using a simple protocol that consists of adding the reagent to a sample, then incubating and centrifuging the mixture. These extracts can be used directly as PCR templates with inhibitor-resistant PCR enzymes.

Cat. #	Product	Size	Price	License	Quantity	Details
9182	MightyPrep Reagent for DNA	20 mL	$114.00
MightyPrep reagent for DNA is designed to easily prepare DNA extracts that can be used directly as PCR templates-from animal tissues (such as mouse tail), plant tissues, blood, processed food, and soil. DNA extracts are prepared using a simple protocol that consists of adding the reagent to a sample, then incubating and centrifuging the mixture. These extracts can be used directly as PCR templates with inhibitor-resistant PCR enzymes such as MightyAmp DNA Polymerase Ver.3 (Cat. #R076A/B)* or Tks Gflex DNA Polymerase (Cat. #R060A/B)* to successfully amplify targets. This product can also be used to efficiently prepare DNA extracts from microorganisms. The resulting extracts can be used as templates with the Bacterial 16S rDNA PCR Kit Fast (800) (Cat. #RR182A)* or the Fungal rDNA (ITS1) PCR Kit Fast (Cat. #RR183A). : Not available in all geographical locations. Check for availability in your region. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Amplification of DNA extracted from various microorganisms using MightyPrep Reagent for DNA Amplification of DNA extracted from various microorganisms using MightyPrep Reagent for DNA. DNA extracts were prepared from colonies of various microorganisms (five bacterial and three fungal species) using MightyPrep reagent for DNA. These bacterial and fungal extracts were then used as templates with PCR kits specially designed for amplifying the bacterial 16S ribosomal DNA (rDNA) region and the fungal ribosomal DNA (rDNA) ITS1 region, respectively. All of the DNA extracts tested were successfully amplified. Lane M1: 250 bp DNA ladder; Lane 1: Salmonella enterica var Enteritidis; Lane 2: Bacillus subtilis; Lane 3: Enterococcus faecalis; Lane 4: Staphylococcus aureus; Lane 5: Escherichia coli; Lane M2: 100 bp DNA ladder; Lane 6: Candida boidinii; Lane 7: Candida tropicalis; Lane 8: Saccharomyces cerevisiae. .

Overview

Simple, rapid protocol
Yields DNA extracts that can be used directly as PCR templates

More Information

Applications

DNA extraction from various microorganisms
DNA extraction from mouse tail
DNA extraction from Salmonella in milk
DNA extraction from soil samples

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Purify DNA from small samples like microdissected tissue, small blood samples, or small amounts of cells.

Designed for extra small samples

Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.

Learn more All genomic DNA kits

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DNA extraction for direct PCR: MightyPrep Reagent for DNA

Notice to purchaser

Amplification of DNA extracted from various microorganisms using MightyPrep Reagent for DNA

Overview

More Information

Applications

Additional product information