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DNA extraction for direct PCR: MightyPrep Reagent for DNA

Easily prepare DNA extracts from animal tissues (such as mouse tail), plant tissues, blood, processed food, and soil for PCR applications

MightyPrep Reagent for DNA is designed to easily prepare DNA extracts from animal tissues (such as mouse tail), plant tissues, blood, processed food, and soil that can be used directly as PCR templates. DNA extracts are prepared using a simple protocol that consists of adding the reagent to a sample, then incubating and centrifuging the mixture. These extracts can be used directly as PCR templates with inhibitor-resistant PCR enzymes. 

Cat. # Product Size License Quantity Details
9182 MightyPrep Reagent for DNA 20 mL USD $139.00

MightyPrep reagent for DNA is designed to easily prepare DNA extracts that can be used directly as PCR templates-from animal tissues (such as mouse tail), plant tissues, blood, processed food, and soil. DNA extracts are prepared using a simple protocol that consists of adding the reagent to a sample, then incubating and centrifuging the mixture. These extracts can be used directly as PCR templates with inhibitor-resistant PCR enzymes such as MightyAmp DNA Polymerase Ver.3 (Cat. #R076A/B)* or Tks Gflex DNA Polymerase (Cat. #R060A/B)* to successfully amplify targets. This product can also be used to efficiently prepare DNA extracts from microorganisms. The resulting extracts can be used as templates with the Bacterial 16S rDNA PCR Kit Fast (800) (Cat. #RR182A)* or the Fungal rDNA (ITS1) PCR Kit Fast (Cat. #RR183A)*.
*: Not available in all geographical locations. Check for availability in your region.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Amplification of DNA extracted from various microorganisms using MightyPrep Reagent for DNA

Amplification of DNA extracted from various microorganisms using MightyPrep Reagent for DNA

Amplification of DNA extracted from various microorganisms using MightyPrep Reagent for DNA. DNA extracts were prepared from colonies of various microorganisms (five bacterial and three fungal species) using MightyPrep reagent for DNA. These bacterial and fungal extracts were then used as templates with PCR kits specially designed for amplifying the bacterial 16S ribosomal DNA (rDNA) region and the fungal ribosomal DNA (rDNA) ITS1 region, respectively. All of the DNA extracts tested were successfully amplified. Lane M1: 250 bp DNA ladder; Lane 1: Salmonella enterica var Enteritidis; Lane 2: Bacillus subtilis; Lane 3: Enterococcus faecalis; Lane 4: Staphylococcus aureus; Lane 5: Escherichia coli; Lane M2: 100 bp DNA ladder; Lane 6: Candida boidinii; Lane 7: Candida tropicalis; Lane 8: Saccharomyces cerevisiae.

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9182: MightyPrep Reagent for DNA

9182: MightyPrep Reagent for DNA

Overviews Genomic DNA kits
Overviews Nucleospin Soil data
Miniprep kits for isolation of genomic DNA from tissue NucleoSpin Tissue

Overview

  • Simple, rapid protocol
  • Yields DNA extracts that can be used directly as PCR templates

More Information

Applications

  • DNA extraction from various microorganisms
  • DNA extraction from mouse tail
  • DNA extraction from Salmonella in milk
  • DNA extraction from soil samples

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Purify DNA from small samples like microdissected tissue, small blood samples, or small amounts of cells.

Designed for extra small samples

Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.

Learn more All genomic DNA kits

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