Fruit-mate for RNA Purification
Plant RNA extraction—which is often difficult using standard RNA extraction kits—can be easily and effectively performed with our Fruit-mate for RNA Purification kit. This kit may be used for plant RNA extraction from a wide variety of plant samples such as leaf, seed, rhizome, fruit, etc., which often contain large amounts of polysaccharides or polyphenols.
Plant RNA extraction—which is often difficult using standard RNA extraction kits—can be easily and effectively performed with our Fruit-mate for RNA Purification kit. This kit may be used for plant RNA extraction from a wide variety of plant samples such as leaf, seed, rhizome, fruit, etc., which often contain large amounts of polysaccharides or polyphenols. Fruit-mate for RNA Purification is ideal for polysaccharide/polyphenol removal because it contains a non-ionic polymer that binds polysaccharides and polyphenols. These substances can then be easily removed from the homogenized sample through a simple centrifugation step.
Overview
- Pre-treatment reagent for total RNA extraction from plants with high polysaccharide or polyphenol content
- Successful results confirmed with a variety of plant samples, including strawberry (fruit), cherry tomato (fruit), banana (fruit), tomato (seed), rice (seed), potato (rhizome), mandarin orange (peel), pine (leaf), aloe (leaf), mango (fruit), and Arabidopsis (seed).
More Information
Applications
Polysaccharide/polyphenol removal from prepared plant RNA
Product citations
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Chen, X.-M. et al. Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol. J. Plant Physiol. 168, 88-95 (2011).
Fujita, K., Horiuchi, H., Takato, H., Kohno, M. & Suzuki, S. Auxin-responsive grape Aux/IAA9 regulates transgenic Arabidopsis plant growth. Mol. Biol. Rep. 39, 7823-9 (2012).
Grant, N. et al. Two cys or not two cys? That is the question; alternative oxidase in the thermogenic plant sacred Lotus. Plant Physiol. 150, 987-95 (2009).
Kimura, S., Nakatsuka, T., Yamada, E., Saito, M. & Nishihara, M. A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian. Plant Biotechnol. 28, 211-221 (2010).
Nakatsuka, T. & Nishihara, M. UDP-glucose:3-deoxyanthocyanidin 5-O-glucosyltransferase from Sinningia cardinalis. Planta 232, 383-92 (2010).
Nakatsuka, T., Saito, M., Yamada, E. & Nishihara, M. Production of picotee-type flowers in Japanese gentian by CRES-T. Plant Biotechnol. 28, 173-180 (2011).
Ono, E. et al. Functional differentiation of the glycosyltransferases that contribute to the chemical diversity of bioactive flavonol glycosides in grapevines (Vitis vinifera). Plant Cell 22, 2856-71 (2010).
Sano, N. et al. Proteomic analysis of embryonic proteins synthesized from long-lived mRNAs during germination of rice seeds. Plant Cell Physiol. 53, 687-98 (2012).
Yamaguchi, H. et al. Phytohormones and willow gall induction by a gall-inducing sawfly. New Phytol. 196, 586-95 (2012).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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