DNA extraction from FFPE samples
DEXPAT reagents are designed for one-step extraction of DNA from paraffin-embedded tissues fixed with 10% formalin (FFPE). The time needed for preparing PCR-ready DNA from paraffin-embedded tissue is dramatically reduced to 25 minutes from the 2–3 days required when using conventional methods.
DEXPAT reagents are designed for one-step extraction of DNA from paraffin-embedded tissues fixed with 10% formalin (FFPE). The time needed for preparing PCR-ready DNA from paraffin-embedded tissue is dramatically reduced to 25 minutes from the 2–3 days required when using conventional methods. To obtain PCR-ready DNA, simply add a paraffin-embedded tissue section to a tube containing DEXPAT Reagent or DEXPAT Easy, incubate it at 100°C, centrifuge at 4°C, and allow to cool on ice for 5 minutes. The refrigerated centrifugation step following heat treatment allows the formation of a gel-like layer above the absorbent resin. When collecting the DNA solution, this gel-like layer prevents contamination of the DNA by the absorbent resin.
DEXPAT Easy retains the performance of DEXPAT Reagent while offering the additional benefits of a ready-to-use reagent dispensed into individual microcentrifuge tubes, as well as an improved composition that facilitates separation of the aqueous DNA solution from the included absorbent resin.
Overview
- No deparaffinization step
- Procedure completed in approximately 30 minutes
- Template-grade DNA (for PCR amplification of DNA <400 bp)
- No toxic substances
- DNA separation from absorbent resin; easy recovery of DNA aqueous solution
More Information
Applications
- DNA extraction from FFPE samples
Materials required but not provided
- Micropipettes
- Refrigerated microcentrifuge
- Block heater (100°C)
- Microtubes (for recovered DNA solution)
- Laboratory gloves
Storage
4°C
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Large-scale, highly pure genomic DNA from cells, tissues, yeast, and bacteria
Due to a specialized manufacturing process that is rigorously controlled and monitored, the beads in NucleoBond AXG columns are uniform in diameter and contain particularly large pores. These special properties allow for optimum flow rates through the column and more efficient binding of nucleic acids to the matrix. Thus, using the matrix you can obtain concentrated genomic DNA with high purity and yields up to 20 µg (AXG 20), 100 µg (AXG 100) or 500 µg (AXG 500).
NucleoBond AXG All kits for genomic DNATakara Bio USA, Inc.
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