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Technical notes and data highlighting the performance of our nucleic acid purification kits Learning center
Home › Products › Cloning › Nucleic acid extraction › Extraction reagents › DEXPAT reagents

Nucleic acid extraction

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DNA extraction from FFPE samples

Fast DNA extraction from FFPE samples

DEXPAT reagents are designed for one-step extraction of DNA from paraffin-embedded tissues fixed with 10% formalin (FFPE). The time needed for preparing PCR-ready DNA from paraffin-embedded tissue is dramatically reduced to 25 minutes from the 2–3 days required when using conventional methods.

DEXPAT reagents are designed for one-step extraction of DNA from paraffin-embedded tissues fixed with 10% formalin (FFPE). The time needed for preparing PCR-ready DNA from paraffin-embedded tissue is dramatically reduced to 25 minutes from the 2–3 days required when using conventional methods. To obtain PCR-ready DNA, simply add a paraffin-embedded tissue section to a tube containing DEXPAT Reagent or DEXPAT Easy, incubate it at 100°C, centrifuge at 4°C, and allow to cool on ice for 5 minutes. The refrigerated centrifugation step following heat treatment allows the formation of a gel-like layer above the absorbent resin. When collecting the DNA solution, this gel-like layer prevents contamination of the DNA by the absorbent resin.

DEXPAT Easy retains the performance of DEXPAT Reagent while offering the additional benefits of a ready-to-use reagent dispensed into individual microcentrifuge tubes, as well as an improved composition that facilitates separation of the aqueous DNA solution from the included absorbent resin.

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Cat. # Product Size License Quantity Details
9104 TaKaRa® DEXPAT™ Easy 50 Rxns USD $157.00 *

TaKaRa DEXPAT Easy is designed for simple and rapid extraction of PCR-ready DNA from Formalin Fixed Paraffin-Embedded (FFPE) tissues. Extraction of DNA from paraffin-embedded tissue sections using conventional methodology is a time-consuming and labor intensive process. TaKaRa DEXPAT Easy, however, offers a rapid and simplified procedure. This reagent retains the performance of TaKaRa DEXPAT (Cat.# 9091), but has the added benefits of being predispensed in reaction tubes and has an improved composition designed to separate the DNA aqueous solution and the absorbent resin during DNA recovery.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

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Comparsion of DNA Extraction Methods using Takara DEXPAT Reagent vs Conventional Method

Comparsion of DNA Extraction Methods using Takara DEXPAT Reagent vs Conventional Method
Comparsion of DNA Extraction Methods using Takara DEXPAT Reagent vs Conventional Method.

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Amplification of the beta-globin gene, using DEXPAT to extract genomic DNA from paraffin sections

Amplification of the beta-globin gene, using DEXPAT to extract genomic DNA from paraffin sections

Amplification of the beta-globin gene

DEXPAT was used to extract genomic DNA from six paraffin sections (each section 10 µm thick) and the DNAs were then used as templates for PCR amplification of the beta-globin gene.

Extracted DNAs were stable at 4°C for at least one month. To achieve accurate and highly sensitive PCR amplification, DNA extracted with DEXPAT is best used in conjunction with Takara Ex Taq DNA polymerase (Cat.# RR001).

PCR reaction mixture:
Extracted genomic DNA5 µl
10X Ex Taq Buffer5 µl
dNTP Mixture (2.5 mM ea.)4 µl
Primer #1 (20 pmol/µl)0.5 µl
Primer #2 (10 pmol/µl)0.5 µl
Takara Ex Taq enzyme0.25 µl
dH2Oup to 50 µl
Thermal cycling conditions:
94°C, 30 sec. |
54°C, 60 sec. |-- 35 cycles
72°C, 60 sec. |
------↓-------
72°C, 5 min: 1 cycle

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9104: TaKaRa DEXPAT Easy

9104: TaKaRa DEXPAT Easy
9091 TaKaRa® DEXPAT™ Reagent 100 Rxns USD $214.00 *

This reagent is designed to enable one-step extraction of PCR-ready DNA from paraffin-embedded tissues fixed in 10% formalin. The one-step extraction protocol eliminates the laborious deparaffinization, organic solvent extraction and enzymatic digestion steps required in standard protocols. DEXPAT Reagent preparation of PCR-ready DNA takes only 25 minutes, versus 2–3 days for conventional extraction methods.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

Comparsion of DNA Extraction Methods using Takara DEXPAT Reagent vs Conventional Method

Comparsion of DNA Extraction Methods using Takara DEXPAT Reagent vs Conventional Method
Comparsion of DNA Extraction Methods using Takara DEXPAT Reagent vs Conventional Method.

Back

Amplification of the beta-globin gene, using DEXPAT to extract genomic DNA from paraffin sections

Amplification of the beta-globin gene, using DEXPAT to extract genomic DNA from paraffin sections

Amplification of the beta-globin gene

DEXPAT was used to extract genomic DNA from six paraffin sections (each section 10 µm thick) and the DNAs were then used as templates for PCR amplification of the beta-globin gene.

Extracted DNAs were stable at 4°C for at least one month. To achieve accurate and highly sensitive PCR amplification, DNA extracted with DEXPAT is best used in conjunction with Takara Ex Taq DNA polymerase (Cat.# RR001).

PCR reaction mixture:
Extracted genomic DNA5 µl
10X Ex Taq Buffer5 µl
dNTP Mixture (2.5 mM ea.)4 µl
Primer #1 (20 pmol/µl)0.5 µl
Primer #2 (10 pmol/µl)0.5 µl
Takara Ex Taq enzyme0.25 µl
dH2Oup to 50 µl
Thermal cycling conditions:
94°C, 30 sec. |
54°C, 60 sec. |-- 35 cycles
72°C, 60 sec. |
------↓-------
72°C, 5 min: 1 cycle

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9091: TaKaRa DEXPAT Reagent

9091: TaKaRa DEXPAT Reagent

Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Mini prep kits to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens NucleoSpin DNA FFPE XS
Two-in-one mini prep kit for the isolation of small (e.g., miRNA) and large RNA from even very limited formalin-fixed, paraffin-embedded samples NucleoSpin totalRNA FFPE

Overview

  • No deparaffinization step
  • Procedure completed in approximately 30 minutes
  • Template-grade DNA (for PCR amplification of DNA <400 bp)
  • No toxic substances  
  • DNA separation from absorbent resin; easy recovery of DNA aqueous solution

More Information

Applications

  • DNA extraction from FFPE samples

Materials required but not provided

  • Micropipettes
  • Refrigerated microcentrifuge
  • Block heater (100°C)
  • Microtubes (for recovered DNA solution)
  • Laboratory gloves

Storage

4°C

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Anion exchange columns for purification of ultra-pure genomic DNA from tissue, bacteria, or yeast by gravity flow

Large-scale, highly pure genomic DNA from cells, tissues, yeast, and bacteria

Due to a specialized manufacturing process that is rigorously controlled and monitored, the beads in NucleoBond AXG columns are uniform in diameter and contain particularly large pores. These special properties allow for optimum flow rates through the column and more efficient binding of nucleic acids to the matrix. Thus, using the matrix you can obtain concentrated genomic DNA with high purity and yields up to 20 µg (AXG 20), 100 µg (AXG 100) or 500 µg (AXG 500).

NucleoBond AXG All kits for genomic DNA

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