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Home › Learning centers › Gene function › Gene editing › Genome-wide screening

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Genome-wide phenotypic screening using CRISPR/Cas9

CRISPR/Cas9 is very well suited for genome-wide knockout screens due to the ease of generating guide RNAs and the specificity of Cas9-sgRNA complexes to completely knock out expression of target genes.

Phenotypic screening using CRISPR technology is not as daunting as it seems. In the "What you need to know about CRISPR library screening" section of our FAQs page, our gene editing scientists have summarized all the steps involved, provided tips, and compiled an exhaustive list of answers to the questions we regularly receive about phenotypic screening with our genome-wide sgRNA library system. Get answers to all of these questions and more:

  • What are the steps for performing a genome-wide sgRNA library screen?
  • How is a positive screen different from a negative screen?
  • Why are there >76,000 different guides in a library?
  • Which cells should I use, and how many?
  • Is Cas9 expression level important?
  • Why do you recommend four guides per target and not eight?

Everything you need to know about CRISPR library screening

A comprehensive list of answers to the questions we receive about phenotypic screening using genome-wide sgRNA libraries.

FAQs and more

Genome-wide sgRNA library screening made simple.

Watch this webinar to learn how you can perform genome-wide lentiviral sgRNA screens easily.

Hear about design and execution of genome-wide CRISPR-based knockout screens

Performing a phenotypic screen using the Guide-it CRISPR Genome-Wide sgRNA Library System

Streamlined knockout screening with a system that provides simplified lentiviral delivery of Cas9 and 76,000+ sgRNAs.

View data on a phenotypic screen for 6TG resistance using our sgRNA library system


Lentiviral CRISPR/Cas9 knockout screening made simple

In this webinar, Thomas Quinn, M.S. (Takara Bio USA, Inc.) discusses the design and execution of genome-wide CRISPR/Cas9-based knockout screens using pooled sgRNA libraries. He also describes features and benefits of the Guide-it CRISPR Genome-Wide sgRNA Library System and NGS analysis kit, a complete solution developed with the aim of making lentiviral CRISPR/Cas9 screening accessible to all.

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Lentiviral CRISPR/Cas9 knockout screening made simple

In this webinar, Thomas Quinn, M.S. (Takara Bio USA, Inc.) discusses the design and execution of genome-wide CRISPR/Cas9-based knockout screens using pooled sgRNA libraries. He also describes features and benefits of the Guide-it CRISPR Genome-Wide sgRNA Library System and NGS analysis kit, a complete solution developed with the aim of making lentiviral CRISPR/Cas9 screening accessible to all.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
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  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
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  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
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  • Gene function
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  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
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  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
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