CRISPR/Cas9 is very well suited for genome-wide knockout screens due to the ease of generating guide RNAs and the specificity of Cas9-sgRNA complexes to completely knock out expression of target genes.
Phenotypic screening using CRISPR technology is not as daunting as it seems. In the "What you need to know about CRISPR library screening" section of our FAQs page, our gene editing scientists have summarized all the steps involved, provided tips, and compiled an exhaustive list of answers to the questions we regularly receive about phenotypic screening with our genome-wide sgRNA library system. Get answers to all of these questions and more:
A comprehensive list of answers to the questions we receive about phenotypic screening using genome-wide sgRNA libraries.
Watch this webinar to learn how you can perform genome-wide lentiviral sgRNA screens easily.
Hear about design and execution of genome-wide CRISPR-based knockout screens
Streamlined knockout screening with a system that provides simplified lentiviral delivery of Cas9 and 76,000+ sgRNAs.
View data on a phenotypic screen for 6TG resistance using our sgRNA library system
In this webinar, Thomas Quinn, M.S. (Takara Bio USA, Inc.) discusses the design and execution of genome-wide CRISPR/Cas9-based knockout screens using pooled sgRNA libraries. He also describes features and benefits of the Guide-it CRISPR Genome-Wide sgRNA Library System and NGS analysis kit, a complete solution developed with the aim of making lentiviral CRISPR/Cas9 screening accessible to all.
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Lentiviral CRISPR/Cas9 knockout screening made simple
In this webinar, Thomas Quinn, M.S. (Takara Bio USA, Inc.) discusses the design and execution of genome-wide CRISPR/Cas9-based knockout screens using pooled sgRNA libraries. He also describes features and benefits of the Guide-it CRISPR Genome-Wide sgRNA Library System and NGS analysis kit, a complete solution developed with the aim of making lentiviral CRISPR/Cas9 screening accessible to all.
