Genome-wide phenotypic screening using CRISPR/Cas9
CRISPR/Cas9 is very well suited for genome-wide knockout screens due to the ease of generating guide RNAs and the specificity of Cas9-sgRNA complexes to completely knock out expression of target genes.
Phenotypic screening using CRISPR technology is not as daunting as it seems. In the "What you need to know about CRISPR library screening" section of our FAQs page, our gene editing scientists have summarized all the steps involved, provided tips, and compiled an exhaustive list of answers to the questions we regularly receive about phenotypic screening with our genome-wide sgRNA library system. Get answers to all of these questions and more:
- What are the steps for performing a genome-wide sgRNA library screen?
- How is a positive screen different from a negative screen?
- Why are there >76,000 different guides in a library?
- Which cells should I use, and how many?
- Is Cas9 expression level important?
- Why do you recommend four guides per target and not eight?
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