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Creating and screening for SNPs
One of the most powerful applications of genome editing technology involves the introduction of single-nucleotide substitutions at specific genomic sites to mimic single-nucleotide polymorphisms (SNPs) implicated in human diseases or to introduce stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single-nucleotide edit remains a challenge, especially in the absence of a phenotype. The Guide-it Knockin Screening Kit provides the ability to quickly identify edited clones in 96-well plates, employing a simple and rapid workflow that comprises PCR amplification of the genomic target site followed by an enzymatic assay with a fluorescent readout. Click on the links below to learn more.
SNP engineering with the Guide-it Knockin Screening Kit
Learn about a simple assay for sensitive detection of single-nucleotide substitutions in bulk-edited or clonal cell populations.
Oligo design tool for Guide-it SNP screening assays
Use our simple online tool to rapidly design assay-specific oligos for SNP detection with the Guide-it Knockin Screening Kit.
Streamlined SNP engineering in iPSCs with the Guide-it Knockin Screening Kit
In this on-demand webinar, Dr. Montse Morell discusses the challenges associated with engineering SNPs in iPSCs and a rapid, user-friendly assay she developed to help overcome these challenges.
Guide-it SNP Screening Kit FAQs
Get answers to frequently asked questions and view a video explaining the enzymatic assay.
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