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Streamlined SNP engineering in iPSCs with the Guide-it Knockin Screening Kit
By enabling the introduction of precise single-nucleotide changes at virtually any locus, CRISPR/Cas genome editing technology has dramatically expanded the capacity to generate model systems relevant to the study of human disease. However, these applications often rely on an endogenous cellular mechanism known as homology-directed repair (HDR) that occurs at relatively low frequencies in iPSCs. Consequently, reliable detection of precise editing in mixed populations and identification of successfully edited clones can prove challenging for researchers.
In this on-demand webinar, Dr. Montse Morell discusses the challenges associated with engineering SNPs in iPSCs, and presents a novel assay that she developed to enable rapid, reliable screening for precise single-nucleotide edits in bulk-edited or clonal populations.
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