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Gene editing of immune cells
A powerful tool for basic and clinical researchers is the ability to genetically modify immune cells using targeted genome engineering approaches such as CRISPR/Cas9 technology. However, inefficient delivery methods and low expression of CRISPR-Cas9 components in T cells have made the application of this technology to primary human immune cells a challenge. Using viral vectors or plasmids to apply CRISPR-Cas9 for genome editing in T cells results in low targeting efficiency and high toxicity. These issues, however, can be overcome by using electroporation to deliver Cas9 ribonucleoprotein complexes (RNPs) in tandem with single-stranded DNA templates for homology-directed repair (HDR). To help our customers circumvent some of these challenges, we share protocols developed by our R&D team for efficient editing of CD3+ Pan T cells and CD34+ cells using electroporation to deliver Cas9 RNPs and ssDNA templates.
User-generated protocols are based on internal proof-of-concept experiments, customer collaborations, and published literature. In some cases, relevant results are discussed in our research news BioView blog articles. While we expect these protocols to be successful in your hands, they may not be fully reviewed or optimized. We encourage you to contact us or refer to the published literature for more information about these user-generated and -reported protocols.
If you are looking for a product-specific, fully optimized User Manual or Protocol-At-A-Glance, please visit the product's product page, open the item's product details row in the price table, and click Documents. More detailed instructions for locating documents are available on our website FAQs page.
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