A fast and reliable method for SNP screening in genome editing experiments
A popular application of CRISPR/Cas genome editing technology involves engineering single-nucleotide substitutions via homology-directed repair (HDR). However, this method can be highly inefficient, causing researchers to sequence hundreds of clones or perform enrichment steps that require additional genomic manipulations—all before confirming that HDR occurred successfully.
In this webinar, Montse Morell, PhD (Takara Bio USA, Inc.) presents a kit she developed to enable rapid, accurate detection of single-nucleotide substitutions and confirmation of successful HDR in edited cells. She also demonstrates an application of the kit in the development of disease models using iPSCs.
About the presenter
Montse Morell, PhD
Scientist III (Cell biology)
Dr. Morell received her BS degree with honors in Chemistry from Universitat Autonoma de Barcelona in 2003. In 2008 she completed her PhD in Biotechnology at the same university. Her thesis work focused on the development of protein reporters to study in vivo protein interactions and aggregation. Afterwards, she performed her postdoctoral work at Stanford University where she developed fluorescent probes to detect the activity of metaloproteases in vivo. Since 2013 she has been working at Takara Bio USA, Inc. as a scientist and has played an instrumental role in the development of products related to genome engineering using CRISPR/Cas9.
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