The pre-linearized template vector contains the T7 promoter, transcription initiation sequence (AGG), 5′-UTR (untranslated region), 3′-UTR, and poly(A) sequence (Figure 1).
Because the Linearized Template Vector has the transcription start site “AGG” as a default sequence, it is compatible with CleanCap Reagent AG (TriLink BioTechnologies, No. N-7113 or N-7413, not included). This allows for convenient, co-transcriptional capping.
The Linearized Template Vector also has 15 bp of In-Fusion cloning sites at the end of 5′-UTR and at the beginning of 3′-UTR, where your GOI can be inserted (Figure 2). Download sequence information for the Linearized Template Vector (fasta format).