Takara IVTpro mRNA Synthesis Systems
The Takara IVTpro mRNA Synthesis Systems (Cat. # 6141, 6148 & 6131) offer a complete solution for synthesizing high-quality single-stranded mRNA from your gene of interest, with a cap structure (using CleanCap Reagent AG from TriLink Biotechnology or similar, not included) and poly(A) sequence. They contain:
- Cloning Kit for mRNA Template (Cat. # 6143 or 6133)—enables easy cloning of a template DNA into a pre-designed In-Fusion cloning-ready plasmid system for in vitro transcription with the Takara IVTpro T7 mRNA Synthesis Kit. The plasmid supplied with Cat. # 6143 contains a Hind III site directly after the poly(A) sequence, while Cat. # 6133 contains a BspQ I site directly after the poly(A) sequence.
- Takara IVTpro T7 mRNA Synthesis Kit (Cat. # 6144 or 6134)—includes a highly efficient T7 RNA polymerase mix optimized for preparing high yields of mRNA through in vitro transcription. The kit also allows integration with capping systems (not included) and RNA modification with modified NTPs. The Enzyme Mix provided as part of Cat. # 6134 contains PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production.
Overview
Takara IVTpro mRNA Synthesis Systems accelerate RNA-focused research with the following benefits:
- Streamlined workflow—complete in vitro transcription workflow, from template preparation to mRNA purification, with a single kit
- Ultra-high yield—generate up to 200 µg of mRNA per reaction with a cap structure and poly(A) sequence
- Flexibility—incorporate a wide variety of capping systems, including CleanCap or ARCA and/or a modified nucleoside such as pseudouridine
More Information
Applications
- Construction of plasmid DNA for in vitro transcription template
- Capped mRNA synthesis using TriLink's CleanCap Reagent AG, or other cap analogs such as Anti-Reverse Cap Analog (ARCA)*
- Synthesis of long non-coding RNA or siRNA precursors for non-coding RNA research
- Synthesis of RNA standard template for RT-qPCR
- RNA probe synthesis for hybridization
A different template sequence design is required when using ARCA.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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mRNA synthesis learning center
Explore Takara IVTpro products and learn how our technology can support your mRNA therapeutic research. This learning center includes vector information, different 5′-capping compatibility, a link to download a flyer, and more.
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T7 RNA Polymerase
The enhanced activity of T7 RNA Polymerase ver.2.0 enables the production of large quantities of high-quality RNA with high processivity and transcription frequency.
Inorganic Pyrophosphatase
Use our inorganic pyrophosphatase to improve the RNA yield in in vitro transcription reactions.
Vaccinia Capping Enzyme
Vaccinia Capping Enzyme adds Cap-0 structures to the 5′ ends of uncapped, in vitro-transcribed RNA.
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