Multiple tissue cDNA panels
MTC multiple tissue cDNA panels are sets of first-strand cDNAs from 5 to 12 different tissues or cell lines. They offer fast and accurate PCR analysis of gene expression across multiple tissues in a fraction of the time required for Northern or dot blot analysis. Each MTC panel has been normalized to several different housekeeping genes to ensure accurate assessment of target mRNA abundance. In addition, human panels are normalized against one another, allowing comparison of results obtained using different panels. MTC panels are virtually free of genomic DNA, ensuring all signals generated are from actual mRNA transcripts.
MTC multiple tissue cDNA panels are sets of first-strand cDNAs from 5 to 12 different tissues or cell lines. They offer fast and accurate PCR analysis of gene expression across multiple tissues in a fraction of the time required for Northern or dot blot analysis. Each MTC panel has been normalized to several different housekeeping genes to ensure accurate assessment of target mRNA abundance. In addition, human panels are normalized against one another, allowing comparison of results obtained using different panels. MTC panels are virtually free of genomic DNA, ensuring all signals generated are from actual mRNA transcripts.
In addition to two broad-coverage human cDNA panels, we offer human digestive system, immune system, and cell line-specific panels. For rapid screening of gene expression under different apoptotic conditions we provide an MCF7 Apoptosis cDNA Panel, consisting of first-strand cDNAs synthesized from total RNA of cells treated with various apoptosis-inducing agents. The MCF7 cDNA Panel, designed for studies focusing on p53-regulated apoptosis, contains cDNA from modified and unmodified MCF7 cells expressing dominant-negative p53.
Overview
- Ready for quantitative PCR
- Quickly determine the tissue distribution and relative abundance of specific transcripts
- Accurate and sensitive expression analysis
- Free of genomic DNA, ensuring that all signals generated are from mRNA transcripts
More Information
Components
| Premium RNA sources for MTC panels | |||
|---|---|---|---|
| Human I (Cat. # 636742) |
Human II (Cat. # 636743) |
Human digestive system (Cat. # 636746) |
Human immune system (Cat. # 636748) |
| Heart Brain (whole) Placenta Lung Liver Skeletal muscle Kidney Pancreas |
Spleen Thymus Prostate Testis Ovary Small intestine Colon Peripheral blood leukocyte |
Cecum Ascending colon Descending colon Transverse colon Duodenum Esophagus Ileocecum Jejunum Ileum Liver Rectum Stomach |
Spleen Lymph node Thymus Tonsil Peripheral blood leukocyte Bone marrow Liver |
| Human cell line (Cat. # 636753)* |
Mouse I (Cat. # 636745) |
Mouse II (Cat. # 636757)* |
Rat (Cat. # 636751) |
| HEK 293 SKOV-3 Saos-2 A-431 Du145 H1299 HeLa MCF7 |
Heart Brain Spleen Lung Liver Skeletal muscle Kidney Testis 7-, 11-, 15-, and 17-day embryos |
Placenta Eye Lymph node Smooth muscle Prostate Thymus Uterus Stomach |
Heart Brain Spleen Lung Liver Skeletal muscle Kidney Testis |
Note: The RNA source may vary from lot to lot. For current source information, please refer to the Certificate of Analysis accompanying each MTC panel.
*The indicated panels were generated from total RNA starting material. All other MTC panels have been prepared using cDNA generated from poly A+ RNA.
Applications
- Detect mRNA present at all abundance levels—including rare transcripts that are difficult to detect using Northern blots
- Expression profiling by qPCR
- Determine the differences in gene expression between tissues
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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