Reverse transcription and cDNA synthesis kit

The Advantage RT-for-PCR Kit allows first-strand cDNA synthesis from either total or poly A⁺ RNA. With this kit, you can obtain sufficient quantities of first-strand cDNA for PCR from nanogram quantities of any RNA. The Advantage RT-for-PCR Kit offers several advantages, including longer oligo(dT) primers for more effective priming of cDNA synthesis, and an RNase inhibitor to minimize RNA degradation. Each kit contains everything you need for cDNA synthesis, including Oligo(dT)₁₈ and random hexamer primers for cDNA synthesis, PCR control primers, and control RNA. This kit does not include reagents needed for PCR. We recommend Titanium Taq DNA Polymerase for sensitive, robust performance in PCR applications.

Cat. #	Product	Size
639506	Advantage® RT-for-PCR Kit	100 Rxns	USD $732.00
Complete kit for the synthesis of first-strand cDNA from total RNA or polyA⁺ RNA. The kit contains sufficient reagents for 100 first-strand cDNA synthesis reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit. The time course of lipopolysaccharide induction of iNOS mRNA in the mouse macrophage cell line RAW 264.7 was studied by isolating RNA from culture samples taken at the time points indicated (in hr). Approx. 0.4 μg was then reverse transcribed using the Advantage RT-for-PCR Kit, and 5% of the cDNA product was used as a template for PCR using the Mouse G3PDH Amplimer Set (Cat. # 639009) as a control (Panel A) and mouse iNOS primers (Panel B). 20% portions of the PCR products were run on a 1.8% EtBr/agarose gel. Lane M: ΦX174/Hae III DNA size marker. Back PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors. The indicated amounts of Human Lung Total RNA (Cat. # 636507) were used for reverse transcription, and 5% of each cDNA product was amplified by PCR using the Human G3PDH Control Amplimer Set (Cat. # 639005). Lanes 1, 5 & 9: 250 ng. Lanes 2, 6 & 10: 62.5 ng. Lanes 3, 7 & 11: 16 ng. Lanes 4, 8 & 12: 4 ng. Lane M: Φ X174/Hae III DNA size marker. The results show that when 4 ng of RNA was used as the starting material, only the Advantage RT-for-PCR Kit generated a clearly visible product. Back 639506: Advantage RT-for-PCR Kit
639505	Advantage® RT-for-PCR Kit	25 Rxns	USD $370.00
Complete kit for the synthesis of first-strand cDNA from total RNA or polyA⁺ RNA. The kit contains sufficient reagents for 25 first-strand cDNA synthesis reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit. The time course of lipopolysaccharide induction of iNOS mRNA in the mouse macrophage cell line RAW 264.7 was studied by isolating RNA from culture samples taken at the time points indicated (in hr). Approx. 0.4 μg was then reverse transcribed using the Advantage RT-for-PCR Kit, and 5% of the cDNA product was used as a template for PCR using the Mouse G3PDH Amplimer Set (Cat. # 639009) as a control (Panel A) and mouse iNOS primers (Panel B). 20% portions of the PCR products were run on a 1.8% EtBr/agarose gel. Lane M: ΦX174/Hae III DNA size marker. Back PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors. The indicated amounts of Human Lung Total RNA (Cat. # 636507) were used for reverse transcription, and 5% of each cDNA product was amplified by PCR using the Human G3PDH Control Amplimer Set (Cat. # 639005). Lanes 1, 5 & 9: 250 ng. Lanes 2, 6 & 10: 62.5 ng. Lanes 3, 7 & 11: 16 ng. Lanes 4, 8 & 12: 4 ng. Lane M: Φ X174/Hae III DNA size marker. The results show that when 4 ng of RNA was used as the starting material, only the Advantage RT-for-PCR Kit generated a clearly visible product. Back 639505: Advantage RT-for-PCR Kit

Overview

Provides efficient first-strand cDNA synthesis from nanogram quantities of RNA in just one hour
Allows simultaneous analysis of multiple genes from a single RNA source
Generates more full-length cDNA; recombinant RNase inhibitor included

More Information

Applications

Two-step RT-PCR
Gene expression analysis
First-strand cDNA synthesis
Quantitative RT-PCR
RT-PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Reverse transcription and cDNA synthesis kit

Notice to purchaser

The reliability of cDNA synthesized with the Advantage RT-for-PCR Kit

PCR amplification of cDNA synthesized with the Advantage RT-for-PCR Kit vs. cDNA synthesized with kits from two competitors

639506: Advantage RT-for-PCR Kit