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Purified cDNA & genomic DNA

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RACE-ready cDNA: rat

Marathon-Ready cDNA—made from high-quality poly A+ RNA and ligated to the Marathon Adaptor—is ready for 5'- and 3'-RACE PCR (Borson, Salo, and Drewes 1992). Marathon-Ready cDNA is a tissue-specific "pool" of premade double-stranded cDNA from which full-length transcript sequences can be amplified using gene-specific primers. It can also be used to study tissue-specific gene expression and to find polymorphic forms of mRNA, or mRNA belonging to a multigene family.

This Marathon-Ready cDNA is synthesized from premium RNA derived from various rat tissues using a procedure optimized to produce full-length cDNA and to eliminate 3' heterogeneity (Chenchik, A., Diachenko, L., Moqadam, F., Tarabykin, V., Lukyanov, S., and Siebert 1996). After synthesis, blunt ends are created and the Marathon Adaptor is ligated to both ends of the double-stranded cDNA.

Cat. # Product Size License Quantity Details
639417 Rat Testis Marathon®-Ready cDNA 30 Rxns USD $921.00

Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5' and 3' Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-μl Marathon RACE reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
639208 Titanium® Taq DNA Polymerase 100 Rxns USD $178.00

Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStart® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. A separate tube of optimized PCR buffer (Mg2+ plus) is supplied as well. Enough enzyme and buffer are supplied for PCR reactions of 50 μl each.

Documents Components Image Data
639412 Rat Brain Marathon®-Ready cDNA 30 Rxns USD $921.00

Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5' and 3' Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-μl Marathon RACE reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Required Products

Cat. # Product Size Price License Quantity Details
639208 Titanium® Taq DNA Polymerase 100 Rxns USD $178.00

Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStart® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. A separate tube of optimized PCR buffer (Mg2+ plus) is supplied as well. Enough enzyme and buffer are supplied for PCR reactions of 50 μl each.

Documents Components Image Data

Overview

  • Adaptor-ligated and ready for RACE PCR
  • Tissue-specific "pool" of double-stranded cDNA
  • Obtain full-length cDNAs without ever constructing or screening a cDNA library
  • Perfect template for Marathon cDNA amplification

More Information

Applications

  • Rapid amplification of cDNA ends (RACE)
  • Study tissue-specific gene expression
  • Discover polymorphic forms of mRNA
  • Obtain full-length copies of published cDNAs
  • Characterization of tissue-specific patterns of gene expression

Source

For relevant source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.

References

Borson, N. D., Salo, W. L. & Drewes, L. R. A lock-docking oligo(dT) primer for 5' and 3' RACE PCR. PCR Methods Appl. 2, 144-8 (1992).     

Chenchik, A., Diachenko, L., Moqadam, F., Tarabykin, V., Lukyanov, S., and Siebert, P. D. Full-Length cDNA Cloning and Determination of mRNA 5' and 3' Ends by Amplification of Adaptor-Ligated cDNA. Biotechniques 21, 526-534 (1996).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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