6141
Takara IVTpro™ mRNA Synthesis System
1 Set
USD $437.00
Takara IVTpro mRNA Synthesis System includes all components necessary from DNA template preparation to high-yield in vitro transcription reaction. Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield. This kit includes the Cloning Kit for mRNA Template (Cat. # 6143) and Takara IVTpro mRNA Synthesis Kit (Cat. # 6144), which are also sold separately.
Notice to purchaser
This product is for in vitro diagnostic use. Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from Takara Bio Inc. If you require licenses for other use, please contact us by phone at +81 77 .565. 6976 or from our website at https://www.takara-bio.co.jp/inquiry/ivd/ . Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements. All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.
6143
Cloning Kit for mRNA Template
10 Rxns
USD $196.00
Cloning Kit for mRNA Template enables easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions. The pre-linearized plasmid contains the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs (untranslated region), and poly(A) sequence. The kit also includes an In-Fusion Snap Assembly Master Mix for seamless cloning of the target DNA into the pre-linearized vector and a FLuc Control Fragment, consisting of 15 bp 3' and 5' In-Fusion sequences and Fluc CDS (1,683 bp).
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
6144
Takara IVTpro™ T7 mRNA Synthesis Kit
20 Rxns
USD $273.00
Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription using an optimized, highly efficient T7 RNA polymerase. The kit includes separate NTPs for easy optimization or replacement with modified nucleosides, such as pseudouridine. The kit synthesizes up to 200 μg or more of mRNA per 20 μl reaction, includes DNase I to digest the template DNA after the in vitro transcription reaction and a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications.
The kit also includes a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs, FLuc CDS, and poly(A) sequence.
Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
2460A
Vaccinia Capping Enzyme
500 U
USD $190.00
Vaccinia Capping Enzyme adds 7-methylguanylate cap structures (Cap-0) to the 5’ ends of uncapped RNA. Vaccinia Capping Enzyme (10 U/µl), 10X Capping Buffer, S-adenosylmethionine (SAM), and GTP are included.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
2460B
Vaccinia Capping Enzyme
2,000 U
USD $698.00
Vaccinia Capping Enzyme adds 7-methylguanylate cap structures (Cap-0) to the 5’ ends of uncapped RNA. Vaccinia Capping Enzyme (10 U/µl), 10X Capping Buffer, S-adenosylmethionine (SAM), and GTP are included.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
2470A
mRNA Cap 2'-O-Methyltransferase
2500 U
USD $79.00
The mRNA Cap 2'-O-Methyltransferase creates a Cap-1 structure, which promotes translation efficiency, increasing subsequent protein expression. mRNA Cap 2'-O-Methyltransferase (50 U/µl), 10X Capping Buffer, and S-adenosylmethionine (SAM) are included.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
2470B
mRNA Cap 2'-O-Methyltransferase
10,000 U
USD $272.00
The mRNA Cap 2'-O-Methyltransferase creates a Cap-1 structure, which promotes translation efficiency, increasing subsequent protein expression. mRNA Cap 2'-O-Methyltransferase (50 U/µl), 10X Capping Buffer, and S-adenosylmethionine (SAM) are included.
Cat. # 2470B contains 4 of Cat. # 2470A. Please refer to Cat. # 2470A for complete product documentation and resources.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
6148
Takara IVTpro™ mRNA Synthesis System (BspQ Ⅰ)
1 Set
USD $437.00
The Takara IVTpro mRNA Synthesis System (BspQ I) includes all the components necessary to produce high-quality in vitro-transcribed mRNA, from DNA template preparation to the in vitro transcription reaction. This system is supplied with a T7 cloning vector containing a BspQ I restriction site for producing scarless templates for in vitro transcription.
Cat. # 6148 includes the Cloning Kit for mRNA Template (BspQ I) (Cat. # 6133) and Takara IVTpro mRNA Synthesis Kit (Cat. # 6144), both of which are also sold separately.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
6131
Takara IVTpro™ mRNA Synthesis System (low dsRNA)
1 Set
USD $481.00
The Takara IVTpro mRNA Synthesis System (low dsRNA) includes all the components necessary to produce high-quality in vitro-transcribed mRNA, from DNA template preparation to the in vitro transcription reaction. This system is supplied with Enzyme Mix containing PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production.
Cat. # 6131 includes the Cloning Kit for mRNA Template (BspQ I) (Cat. # 6133) and Takara IVTpro mRNA Synthesis Kit (low dsRNA) (Cat. # 6134), both of which are also sold separately.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
6134
Takara IVTpro™ T7 mRNA Synthesis Kit (low dsRNA)
20 Rxns
USD $300.00
The Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription, synthesizing up to 200 μg or more of mRNA per 20 μl reaction.
The kit includes a highly efficient T7 RNA polymerase, separate NTPs for easy optimization or replacement with modified nucleosides, DNase I to digest the template DNA after the in vitro transcription reaction, a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications, and a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs, FLuc CDS, and poly(A) sequence.
Cat. # 6134 has an Enzyme Mix containing PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
6133
Cloning Kit for mRNA Template (BspQ I)
10 Rxns
USD $196.00
Cloning Kit for mRNA Template enables easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions. The kit includes an In-Fusion Snap Assembly Master Mix for seamless cloning of the target DNA into the pre-linearized vector and a FLuc Control Fragment, consisting of 15 bp 3' and 5' In-Fusion sequences and Fluc CDS (1,683 bp).
The pre-linearized plasmid that is provided as part of Cat. # 6144 contains a BspQ I digestion site immediately following the poly(A) sequence. This plasmid can be purchased separately (Cat. #6146 ).
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
