NDiff N2 medium citation list

NDiff N2 medium is a serum-free and chemically defined neural supplement based on Bottenstein's original formulation. NDiff N2 medium can be combined with RHB-BASAL media to enable the culture of a wide variety of neural cell types including neural stem cells, neurons, and neuroblastomas. Read below for a citation list of studies in which NDiff N2 medium was used in peer-reviewed basic, translational, preclinical, and biomedical research.

Bottenstein, J. Cell culture in the neurosciences. (Springer Science & Business Media, 2012).

Conti, L. et al. Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol. 3, e283 (2005).

Conti, L., Reitano, E. & Cattaneo, E. Neural stem cell systems: diversities and properties after transplantation in animal models of diseases. Brain Pathol. 16, 143–54 (2006).

Hook, L. et al. Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays. Neurochem. Int. 59, 432–44 (2011).

McLaren, D. et al. Automated large-scale culture and medium-throughput chemical screen for modulators of proliferation and viability of human induced pluripotent stem cell-derived neuroepithelial-like stem cells. J. Biomol. Screen. 18, 258–68 (2013).

Nichols, J. & Ying, Q.-L. Derivation and propagation of embryonic stem cells in serum- and feeder-free culture. Methods Mol. Biol. 329, 91–8 (2006).

Pollard, S. M., Conti, L., Sun, Y., Goffredo, D. & Smith, A. Adherent neural stem (NS) cells from fetal and adult forebrain. Cereb. Cortex 16 Suppl 1, i112–20 (2006).

Ying, Q.-L. & Smith, A. G. Defined conditions for neural commitment and differentiation. Methods Enzymol. 365, 327–41 (2003).

Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183–6 (2003).