- Cellartis Cardiomyocytes (from ChiPSC22) Kit
- Cellartis Cardiomyocytes (from ChiPSC22)
- Cellartis CM Thawing Base
- Cellartis CM Culture Base
- Fetal Bovine Serum (FBS) (Thermo Fisher Scientific, Cat. # 16140063)
- Cell culture T25 flask(s)
- Fibronectin (Sigma-Aldrich, Cat. # F0895)
- Stopping medium (i.e. any medium w serum, w/o antibiotics)
- External solution (140 mM NaCl, 10 mM HEPES, 5 mM Glucose, 4 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 298 mOsm, pH 7.4)
- Sterile H2O
- Pluripotent stem cells
Preparing cardiomyocytes for the Patchliner system
Cellartis cardiomyocytes are derived from human induced pluripotent stem cells and provide a promising physiologically-relevant, human model for pre-clinical testing and drug screening. Cellartis cardiomyocytes together with the throughput, performance, and versatility of the Patchliner system provide a powerful combination for making more predictive cardiac disease models, and for accurately predicting cardiotoxic responses.
The following protocol has been optimized to detach Cellartis Cardiomyocytes (from ChiPSC22) for optimal use in the Patchliner system (Nanion). To prepare cardiomyocytes for recordings with the Patchliner's automatic patch clamp, the cultured cells need to be harvested in a gentle way with minimal disruption of epitopes or cell morphology. Use one fibronectin T25 flask with Cellartis Cardiomyocytes (from ChiPSC22) as a starting material.
User-generated protocols are based on internal proof-of-concept experiments, customer collaborations, and published literature. In some cases, relevant results are discussed in our research news BioView blog articles. While we expect these protocols to be successful in your hands, they may not be fully reviewed or optimized. We encourage you to contact us or refer to the published literature for more information about these user-generated and -reported protocols.
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A highly homogeneous population of cardiomyocytes derived from human induced pluripotent stem cells.
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