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  • ‹ Back to Cardiomyocytes
  • Cardiomyocytes in FLIPR 384-well plate format
  • Cardiomyocytes on the Patchliner system
  • Cardiomyocytes on the Maestro MEA system
  • Cardiomyocytes on the MED64 MEA system
  • Cardiomyocytes on the CardioExcyte 96 system
  • Cardiomyocytes on the xCELLigence RTCA CardioECR system
Citations Cellartis hiPS-CM citation list
Home › Learning centers › Stem cell research › Protocols › Cardiomyocytes › Cardiomyocytes on the MED64 MEA system

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Citations Cellartis hiPS-CM citation list
User-generated protocol

Cardiomyocytes on the MED64 MEA system

Cellartis cardiomyocytes are derived from human induced pluripotent stem cells and provide a promising, physiologically relevant, human model for preclinical testing and drug screening. Alpha MED Scientific's MED64 platform allows for noninvasive detection of electrophysiological parameters such as beat frequency and field potential duration (FPD). Cellartis cardiomyocytes used in combination with the MED64 platform demonstrate the potential to accurately predict cardiotoxic responses and to screen compound efficacy.

Materials required Protocol

Materials required  

  •  Cellartis Cardiomyocytes (from ChiPSC22) Kit (Cat. # Y10075)
    • Cellartis Cardiomyocytes (from ChiPSC22)
    • Cellartis CM Thawing Base
    • Cellartis CM Culture Base
  • Fetal Bovine Serum (FBS; Thermo Fisher Scientific, Cat. # 16140) 
  • Polyethylenimine (Sigma, Cat. # P3143)
  • Na2B4O7/10H2O (Sigma, Cat. # S9640)
  • Matrigel (Corning, Cat. # 356231)
  • Fibronectin (Sigma-Aldrich, Cat. # F0895) 
  • Y-27632, MF (Wako Pure Chemical Industries, Ltd., Cat. # 257-00613)
  • Trypsin-EDTA (0.25%), phenol red
  • Ethanol (70%)
  • PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)
  • PBS Dulbecco's w/o Ca2+ & Mg2+ (D-PBS –/–)
  • General cell culture equipment used in cell culture laboratory

Protocol  

Cellartis cardiomyocytes, cryopreserved human pluripotent stem cell-derived cardiomyocytes, are initially thawed onto standard tissue culture plates coated with fibronectin. Cells can be plated onto the MED Probe starting three days after thawing. Recordings can be started two days after plating on the MED Probe.

Preparing the MED Probe

NOTE: Electrodes in the MED Probe are extremely fragile. Avoid contact with electrodes in all of the following procedures.

  1. Rinse an MED Probe with sterilized distilled water (SDW) at least three times. Fill the MED Probe with 70% ethanol and leave it for 30 min.
  2. Aspirate the ethanol in a sterile hood.
  3. Fill the MED Probe with 0.1% polyethylenimine (PEI) in 25 mM borate buffer (pH 8.4). Leave it at room temperature overnight. (Make sure the electrodes are covered by the PEI.)
  4. Aspirate the PEI and rinse the MED Probe with double distilled water (DDW) at least 4 times.

    NOTE: PEI coating should be performed each time, before preparing the MED Probe (not just the first time).

  5. Place a dry MED Probe in a sterile 100-mm diameter culture dish.
  6. Place a sterile 35-mm diameter culture dish next to the MED Probe and fill the dish with sterile water to create a moist environment.
  7. Place a 7-μl bead of matrigel (diluted in DPBS +/+ to a final concentration of 200 μg/ml) over the recording electrode area on the MED Probe.
  8. Incubate the matrigel-coated MED Probe in a cell culture incubator at 37°C, with 5% CO2, for 3 hr.

    NOTE: Do not let the matrigel-coated surface dry completely before seeding the cells.

Collecting from tissue culture plates

  1. Make MEA Plating Medium by adding 20% FBS to Cellartis CM Culture Base.
  2. Preheat an appropriate amount of Cellartis MEA Plating Medium to 37°C. Add Y-27632 to a final concentration of 5 μM prior to use.
  3. Aspirate the medium from each well containing cardiomyocytes.
  4. Rinse the wells with DPBS –/–.
  5. Add 0.25% trypsin/EDTA to each well (~80 μl/cm2) and incubate for 2–4 min.
  6. Gently detach the cells by dispensing the dissociation solution over the surface using a 1-ml pipettor.
  7. Add 1 volume (~ 80 μl/cm2) of the MEA Plating Medium (with 5 μM Y-27632) to each well to deactivate the trypsin.
  8. Transfer the cell suspension into a suitable tube.
  9. Count the cardiomyocytes.
  10. Centrifuge the cells at 200g for 5 min at room temperature.
  11. Aspirate the supernatant and gently resuspend the cell pellet in an appropriate volume of the MEA Plating Medium (with 5 μM Y-27632) to a final concentration of 6,000–7,000 viable cardiomyocytes/μl.

Plating onto the MED Probes

  1. Aspirate the matrigel from the MED Probe.
  2. Place a 7-μl bead of the cardiomyocyte suspension (corresponding to 42,000–49,000 viable cardiomyocytes) over the recording electrode area on the MED Probe.

    NOTE: Do not let the matrigel-coated area dry completely before seeding the cells. If seeding Cellartis cardiomyocytes on several MED Probes, we recommended working on one probe at a time.
    CAUTION: The MED Probe has four reference electrodes as well as 64 recording electrodes. The four reference electrodes MUST be free from cells (but covered by medium) for signal recording.

  3. Incubate the MED Probe with the seeded Cellartis cardiomyocytes in a cell culture incubator at 37°C, with 5% CO2, for 2 hr, allowing the cells to adhere.
  4. Gently add 2 ml of the MEA Plating Medium (with 5 μM Y-27632) to the MED Probe, taking care not to dislodge the cells.
  5. Incubate in a cell culture incubator at 37°C, with 5% CO2.
  6. After 24–48 hours, change the medium to Cellartis CM Culture Base + 10% FBS. After this first medium exchange, half-volume medium changes should be performed every two to three days, depending on appearance/pH. Recordings can be started one day after the change to Cellartis CM Culture Base (cardiomyocytes should have started to beat at this point) in the standard assay medium of choice (Cellartis CM Culture Base can be used).

Related Products

Cat. # Product Size Price License Quantity Details
Y10075 Cellartis® Cardiomyocytes (from ChiPSC22) Kit 1 Kit USD $906.00

License Statement

ID Number  
C005 This product is manufactured and sold by Takara Bio Europe AB based on a license to Cellectis SA. The use of this product is strictly limited to purchaser’s own internal research, including basic research, drug discovery research up to and including IND-enabling preclinical toxicological studies or equivalents thereof, excluding any use in the T-cell field. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us. No express or implied license is granted to the purchaser.
L67 This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 9,938,585 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This Product is manufactured and sold by Takara Bio Inc. and/or its affiliate(s) based on a patent license from iPS Academia Japan, Inc.

No express or implied license is granted to the purchaser.

"Product" means the differentiated cells manufactured and sold by Takara Bio Inc.

"Internal research purpose" means the use of this Product as a tool of internal research for the sole benefit of the purchaser or purchaser's contract research customer, (including screening of compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use), provided, however, that the following activities using this Product are strictly prohibited and expressly excluded from Internal research purposes:

With the exemption for academic and non-commercial clinical research done by non-profit institutions;

1) Administration of this Product to humans.

2) Use of this Product for the diagnostic testing, prevention, or treatment of human and/or animal diseases.

3) Use of this Product in studies involving administration to humans for cell therapy, regenerative medicine etc.

Purchaser may use this Product for its own Internal research purposes.

Purchaser shall not use the Products for its commercial purposes.

Notwithstanding the foregoing, the purchaser of this Product may provide third parties with contract research services by using this Product only for such third party's Internal research purposes.

Cellartis Cardiomyocytes (from ChiPSC22) are human cardiomyocytes derived from a human induced pluripotent stem (iPS) cell line. The cells are provided frozen with thawing and maintenance media bases.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

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Cellartis Cardiomyocytes (from ChiPSC22) respond in a dose-dependent manner to hERG blocker E4031 as measured by MEA (MED64 AlphaMED MEA system)

Cellartis Cardiomyocytes (from ChiPSC22) respond in a dose-dependent manner to hERG blocker E4031 as measured by MEA (MED64 AlphaMED MEA system)
Cellartis Cardiomyocytes (from ChiPSC22) respond in a dose-dependent manner to hERG blocker E4031 as measured by MEA (MED64 AlphaMED MEA system). BL = baseline and EAD = early after depolarization.

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Cellartis Cardiomyocytes (from ChiPSC22) express cardiomyocyte markers, including cTroponin (red)

Cellartis Cardiomyocytes (from ChiPSC22) express cardiomyocyte markers, including cTroponin (red)
Cellartis Cardiomyocytes (from ChiPSC22) express cardiomyocyte markers, including cTroponin (red). Nuclei are labelled with DAPI (blue). F-actin is labeled with Phalloidin (green).


User-generated protocols

User-generated protocols

User-generated protocols are based on internal proof-of-concept experiments, customer collaborations, and published literature. In some cases, relevant results are discussed in our research news BioView blog articles. While we expect these protocols to be successful in your hands, they may not be fully reviewed or optimized. We encourage you to contact us or refer to the published literature for more information about these user-generated and -reported protocols. 

If you are looking for a product-specific, fully optimized User Manual or Protocol-At-A-Glance, please visit the product's product page, open the item's product details row in the price table, and click Documents. More detailed instructions for locating documents are available on our website FAQs page.

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Cellartis cardiomyocytes derived from human induced pluripotent stem cells

A highly homogeneous population of cardiomyocytes derived from human induced pluripotent stem cells.

Human stem cell-derived cardiomyocytes


Cellartis hPS cell-derived cardiomyocytes citation list

Publications using Cellartis human pluripotent stem cell-derived cardiomyocytes.

Cellartis hiPS-CM citation list

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