Pluripotent stem cells
At Takara Bio, we are experts in human pluripotent stem cell culture. Whether you want to reprogram fibroblasts or PBMCs to generate human induced pluripotent stem cells (hiPSCs), transfer your hiPSCs from another culture medium or a MEF-based culture system to the Cellartis DEF-CS 500 Culture System, or form spin embryonic bodies to assess hPSC pluripotency, we have protocols to guide you through the process.
View our protocols below:
Single-cell cloning with Cellartis DEF-CS 500 Culture System
Learn how to perform single-cell isolation using the Cellartis DEF-CS 500 Culture System
Transferring iPSCs from other feeder-free culture systems to the DEF-CS culture system
Learn how to adapt iPSCs grown in other feeder-free culture systems to the DEF-CS culture system.
Transferring iPSCs on MEFs to the DEF-CS culture system
Learn how to adapt human iPS cells grown on MEFs to the DEF-CS culture system.
Confirmation of pluripotency by spin embryoid body formation
Learn how to differentiate pluripotent stem cells into embryoid bodies to confirm pluripotency.
Reprogramming fibroblasts using the Cellartis DEF-CS 500 Culture System
Use this step-by-step protocol to reprogram fibroblasts with Sendai virus technology.
Reprogramming peripheral blood mononuclear cells (PBMCs) using the Cellartis DEF-CS 500 Culture System
This application protocol describes step-by-step reprogramming of PBMCs.
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