Cellartis DEF-CS 500 Culture System citation list

Takara Bio has over 15 years of experience in expanding, passaging, and culturing human pluripotent stem cells under the Cellartis brand. The DEF-CS 500 system is a complete solution for the easy culturing of human pluripotent stem cells in a noncolony, 2D-monolayer format. Cells grown in this system maintain pluripotency and a normal karyotype while exhibiting robust and uniform growth. Single-cell passaging enables the ability to directly isolate and expand single clones, including cells that have undergone CRISPR/Cas9 gene editing.

Read below for a citation list of studies in which the DEF-CS 500 system was used in peer-reviewed basic, translational, preclinical, and biomedical research.

• Asplund, A. et al. One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev. 12:90–104 (2016).

25 different human pluripotent stem cell lines were maintained with the DEF-CS system, and their pluripotency was confirmed. Karyotyping of the stem cells maintained using the DEF-CS system for up to 29 passages showed no genetic aberrations. These stem cell lines were then successfully differentiated into functional hepatocytes. 

• Boreström, C. et al. Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: a first step toward a clinical-grade cell source. Stem Cells Transl. Med. 3:433–47 (2014).

Chondrocyte-derived and fibroblast-derived iPSCs were maintained using the DEF-CS system in a monolayer before these cells were successfully differentiated down to the chondrogenic lineage. Pluripotency of iPSCs maintained in the DEF-CS system was confirmed with immunofluorescent staining and quantitative RT-PCR.

• Boreström, C. et al. A CRISP(e)R view on kidney organoids allows generation of an induced pluripotent stem cell-derived kidney model for drug discovery. Kidney Int. 18:30356–9 (2018).

Human induced pluripotent stem cells were maintained, edited with CRISPR/Cas9, and clonally selected in the DEF-CS system. Following clonal expansion, the cells were differentiated using a 3D differentiation protocol to create kidney organoids.

• Delsing, L. et al. Barrier properties and transcriptome expression in human iPSC-derived models of the blood-brain barrier. Stem Cells 36(12):1816–1827 (2018). 

Human induced pluripotent stem cells were maintained in the DEF-CS system prior to differentiation into endothelial cells.

• Funa, N. et al. B-catenin regulates primitive streak induction through collaborative interactions with SMAD2/SMAD3 and OCT4. Cell Stem Cell. 16(6):638–652 (2015).

Human embryonic stem cells were grown in DEF-CS medium prior to differentiation in order to study how β-catenin regulates differentiation. 

• Gao, X. et al. A rapid and highly efficient method for the isolation, purification, and passaging of human induced pluripotent stem cells. Cell Reprogram. 20(5):282–288 (2018). 

Human induced pluripotent stem cells derived from PBMCs that were reprogrammed were single-cell passaged and expanded in DEF-CS medium. Pluripotency and genetic integrity of the hiPSCs were confirmed following single-cell passaging and expansion. 

• Gao, X. et al. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel. Stem Cell Res. 31:193–196 (2018).

Human induced pluripotent stem cells were maintained, passaged as single cells, and expanded in the DEF-CS system. The resulting cell lines showed normal karyotype, expressed pluripotency markers, and were able to differentiate into the three germ layers.

• Ghosheh, N. et al. Comparative transcriptomics of hepatic differentiation of human pluripotent stem cells and adult human liver tissue. Physiol Genomics. 49(8):430–446 (2017).

Human pluripotent stem cells were maintained and passaged in the DEF-CS system prior to differentiation into hepatocytes. Transcriptomics were used to monitor the proress of in vitro hepatic differentiation of hPSCs at the following developmental stages: definitive endoderm, hepatoblast, early hPSC-HEP, and mature hPSC-HEP.

• Ghosheh, N. et al. Highly synchronized expression of lineage-specific genes during in vitro hepatic differentiation of human pluripotent stem cell lines. Stem Cells Int. 2016:8648356 (2016).

Human induced pluripotent stem cells were thawed, maintained, and passaged in the DEF-CS system prior to differentiation into hepatocytes.

• Hanson, C. et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta Ophthalmol. 91(2):127–130 (2013). 

Human embryonic stem cells were cultured in the DEF-CS system prior to transplantation onto a damaged human cornea. 

• Kamiya, A. et al. An in vitro model of polycystic liver disease using genome-edited human inducible pluripotent stem cells. Stem Cell Res. 32:17–24 (2018).

Human induced pluripotent stem cells were maintained in the DEF-CS system prior to differentiation into hepatic progenitor cells, which were used in cytotoxicity assays.

• Kia, R. et al. MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity. Toxicol Sci. 144(1):173–185 (2015).

Human induced pluripotent stem cells were maintained in the DEF-CS system prior to differentiaton into hepatocyte-like cells.

• Mamidi A. et al. Mechanosignalling via integrins directs fate decisions of pancreatic progenitors. Nature. 564(7734):114–118 (2018).

Undifferentiated human embryonic stem cells were maintained in the DEF-CS system prior to differentiation into pancreatic progenitors including bipotent pancreatic progenitors. 

• Nguyen, D. et al. Humanizing miniature hearts through 4-flow cannulation perfusion decellularization and recellularization. Sci Rep. 8(1):7458 (2018).

Human induced pluripotent stem cells were cultured in the DEF-CS system prior to differentiation into cardiac progenitor cells and recellularization experiments.

• Norrman, K. et al. Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59:59–70 (2013).

Human embryonic stem cells were maintained in the DEF-CS system before differentiation into definitive endoderm.

• Osada, N. et al. Lysine-specific demethylase 1 inhibitors prevent teratoma development from human induced pluripotent stem cells. Oncotarget. 9(5):6450–6462 (2018).

Human induced pluripotent stem cells ere cultured in the DEF-CS system prior to gain-of-function and loss-of-function studies.

• Pradip, A. et al. High content analysis of human pluripotent stem cell derived hepatocytes reveals drug induced steatosis and phospholipidosis. Stem Cells Int. 2016:2475631 (2016).

Human induced pluripotent stem cells were maintained in the DEF-CS system prior to differentiation into hepatocytes. hiPSC-derived hepatocytes were treated with various compounds known to cause hepatotoxicity through steatosis and phospholipidosis.

• Rasmussen, C. et al. Collagen type I improves the differentiation of human embryonic stem cells towards definitive endoderm. PLoS One. 10(12):e0145389 (2015).

Human embryonic stem cells were cultured in the DEF-CS system, then exposed to 487 combinations of extracellular matrix proteins to screen for combinations that promote differentiation to definitive endoderm.

• Ribeiro, D. et al. Human pancreatic islet-derived extracellular vesicles modulate insulin expression in 3D-differentiating iPSC clusters. PLoS One. 12(11):e0187665 (2017). 

Human induced pluripotent stem cells were cultured in the DEF-CS system prior to iPS clustering and pancreatic differentiation.

• Säljö, K. et al. Comparison of the glycosphingolipids of human-induced pluripotent stem cells and human embryonic stem cells. Glycobiology. 27(4):291–305 (2017).

Human induced pluripotent stem cells were maintained in the DEF-CS system prior to differentiation into neuroepithelial cells.

• Sivertsson, L.  et al. Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor. Stem Cells Dev. 22(4):581–594 (2012).

Human embryonic stem cells (Y00025) were cultured in the DEF-CS system and differentiated into definitive endoderm cells. The cells were then transferred to a 3D bioreactor and further matured into hepatocyte-like cells.

• Ulvestad, M. et al. Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem Pharmacol. 86(5):691–702 (2013).

Human embryonic stem cells and pluripotent stem cells were maintained and expanded in the DEF-CS system prior to differentiation into hepatocytes. Following differentiation, the researchers characterized the expression and function of important CYP enzymes and transporter proteins in the differentiated cells. 

• Valton, J. et al. Efficient strategies for TALEN-mediated genome editing in mammalian cell lines. Methods. 69(2):151–170 (2014). 

Human induced pluripotent stem cells were maintained in the DEF-CS system before and after transfection by DNA electroporation. 

• Vizlin-Hodzic, D. et al. Early onset of inflammation during ontogeny of bipolar disorder: the NLRP2 inflammasome gene distinctly differentiates between patients and healthy controls in the transition between iPS cell and neural stem cell stages. Transl Psychiatry. 7(1):e1010 (2017).

Human induced pluripotent stem cells were maintained in the DEF-CS system prior to differentiation into neural stem cells.

• Zandén, C. et al. Stem cell responses to plasma surface modified electrospun polyurethane scaffolds. Nanomedicine. 10(5):949–958 (2014).

Human embryonic stem cells (Y00025) were cultured in the DEF-CS system and plated on coverslips that were coated with various plasma surface modified polyurethane scaffolds. The researchers wanted to investigate the effects of the different modifications on stem cell behavior.