Terra PCR Direct Polymerase Mix contains an optimized DNA polymerase that allows for direct amplification from a tissue source or crude extracts, without the need for DNA purification. It can amplify DNA targets up to 4 kb as well as difficult templates (including those with a GC content greater than 70%)—and it enables DNA amplification in the presence of PCR inhibitors, growth serum, and other source-material components.
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- Direct PCR from human nail
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- Amplifying GC-rich templates
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Amplifying a gene of interest from human nail DNA
Terra PCR Direct outperforms competitor PCR enzymes for direct amplification of GC-rich targets and longer (4 kb) targets
- Overview: Terra PCR Direct Polymerase Mix contains an optimized DNA polymerase
- Comparison with competitors’ PCR enzymes: Terra PCR Direct Polymerase demonstrates an exceptional ability to amplify GC-rich targets
Comparison with competitor PCR enzymes
Terra PCR Direct Polymerase Mix demonstrates an exceptional ability to amplify GC-rich targets and relatively long (4 kb) targets from a crude extract containing human nail DNA.
Terra PCR Direct Polymerase Mix (Panel A) was able to successfully amplify all of the samples. The PCR enzyme from Company A (Panel B) was unable to amplify any of the samples, except for slight amplification of one AT-rich sample (Panel B, Lane 4). The PCR enzyme from Company B (Panel C) was unable to amplify any of the GC-rich samples (Panel C, Lanes 3, 6, and 9) and exhibited diminished performance in amplifying targets as target length increased (Panel C, Lanes 2, 5, and 8).
|Amplification results for Terra Direct compared to two other DNA polymerases|
|Lane No.||Target gene||Amplicon size||% GC content||Amplification results|
|Terra PCR Direct||Company A enzyme||Company B enzyme|
*UCRchr9 = Chromosome 9 AT-rich noncoding region.
**UCRchr11 = Chromosome 11 AT-rich noncoding region
This experiment demonstrates that Terra PCR Direct Polymerase is a good choice for analyzing extremely tough, hard-to-lyse tissues such as human nails, which are frequently the subject of forensic analysis. Terra was able to amplify gene fragments from GC-rich templates up to 4 kb in length. In contrast, the polymerase from Company B failed to amplify gene fragments containing over 50% GC, while the polymerase from Company A provided poor or no amplification, except for one AT-rich sample.
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