PCR amplification of GC-rich templates is often hindered by formation of secondary structures and the requirement for high melting temperatures. PrimeSTAR GXL DNA Polymerase is a high-fidelity PCR enzyme that allows efficient amplification of the most challenging templates. With this enzyme, amplification of GC-rich templates is possible without reaction optimization or inclusion of additives such as DMSO or Betaine.
In this experiment, PrimeSTAR GXL DNA Polymerase was compared to other commercially available enzymes for the amplification of a target with high GC content. Only the PrimeSTAR GXL enzyme was able to efficiently amplify the GC-rich template without additives or special reaction conditions.