A fast polymerase for high fidelity PCR—save your sanity as well as your time

Let's face it, there's nothing more frustrating than a failed PCR. When all that stands between you and understanding your gene of interest is DNA amplification, every moment counts. Rely on PrimeSTAR Max DNA Polymerase for cloning and expression, structure-function, and genetic variation studies.

Citation: Yamanaka, K. et al. Rapid detection for primary screening of influenza A virus: microfluidic RT-PCR chip and electrochemical DNA sensor. Analyst 136, 2064-8 (2011).

Experimental example: PrimeSTAR Max vs. other commercially available high-fidelity polymerases using a 10-second extension step

Human genomic DNA was used as template for fast PCR. Products 0.5 to 7.5 kb long were amplified (extension time, 10 sec) using PrimeSTAR Max enzyme or polymerases from three other manufacturers. High yield was observed when PrimeSTAR Max polymerase was used for products up to 4 kb in length.

Compare extension-step recommendations

Is every high-fidelity polymerase capable of fast extension? No. Many high-fidelity polymerases on the market lack sufficient extension speed and are incompatible with fast cycling conditions. Refer to the table below for recommended extension step times for PrimeSTAR Max DNA Polymerase and three other commercially available high-fidelity enzymes.

*See data in Figure 1 for an example of amplification of a 4-kb product with a 10 sec extension step. Routine recommendations: when template quantity is 200 ng/50 µl or less, an extension step of 5 sec/kb is recommended. When template quantity exceeds 200 ng/50 µl, an extension step of 30–60 sec/kb is recommended.

PrimeSTAR Max DNA Polymerase offers the highest fidelity and fastest extension speed of any commercially available polymerase. It was shown to provide superior yields for a series of amplification products up to 4 kb in length when compared to polymerases from other manufacturers. PrimeSTAR Max DNA Polymerase also requires a far shorter extension time than other commercially available high-fidelity polymerases, making it a better choice for high-fidelity PCR performed under fast cycling conditions.