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  • Rapid, high-performance multiplex PCR
  • EmeraldAmp outperforms MyTaq Red mix
  • Fast and accurate PCR
  • Methylation studies
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  • Titanium Taq for high-throughput genotyping
  • Colony PCR in under an hour
  • High-throughput endpoint PCR
  • Direct PCR from blood
  • PrimeSTAR GXL for targeted sequencing
  • Detecting somatic mosaicism using massively parallel sequencing
standard pcr PCR Amplification Kit
SpeedSTAR, hot start DNA Polymerase SpeedSTAR, hot start
Home › Learning centers › PCR › Technical notes › Fast and accurate PCR

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    • Detecting somatic mosaicism using massively parallel sequencing
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standard pcr PCR Amplification Kit
SpeedSTAR, hot start DNA Polymerase SpeedSTAR, hot start
Tech Note

Go from DNA to data with a fast polymerase that's also astonishingly accurate

PrimeSTAR Max DNA Polymerase

  • Highest fidelity of any commercially available polymerase
  • Fastest extension speed available
  • Formulated as a hot-start premix with dNTPs and buffer: just add primers, DNA, bring to final volume with dH2O, and go.

Introduction Results Conclusions

Introduction  

A fast polymerase for high fidelity PCR—save your sanity as well as your time

Let's face it, there's nothing more frustrating than a failed PCR. When all that stands between you and understanding your gene of interest is DNA amplification, every moment counts. Rely on PrimeSTAR Max DNA Polymerase for cloning and expression, structure-function, and genetic variation studies.

Fast polymerase PrimeSTAR Max

Using a fast polymerase to detect influenza. Scientists at Osaka University developed a microfluidic device for rapid detection of influenza A (shown in color in the figure above). Using a mix of PrimeScript  RTase, PrimeSTAR Max polymerase, and SpeedSTAR polymerase, RT-PCR was completed in 15 minutes.

Citation: Yamanaka, K. et al. Rapid detection for primary screening of influenza A virus: microfluidic RT-PCR chip and electrochemical DNA sensor. Analyst 136, 2064-8 (2011).

Results  

Experimental example: PrimeSTAR Max vs. other commercially available high-fidelity polymerases using a 10-second extension step

Human genomic DNA was used as template for fast PCR. Products 0.5 to 7.5 kb long were amplified (extension time, 10 sec) using PrimeSTAR Max enzyme or polymerases from three other manufacturers. High yield was observed when PrimeSTAR Max polymerase was used for products up to 4 kb in length.

fast polymerase data

Figure 1. Fast PCR amplification of human genomic DNA using PrimeSTAR Max DNA Polymerase or high-fidelity polymerases provided by other manufacturers.

Compare extension-step recommendations

Is every high-fidelity polymerase capable of fast extension? No. Many high-fidelity polymerases on the market lack sufficient extension speed and are incompatible with fast cycling conditions. Refer to the table below for recommended extension step times for PrimeSTAR Max DNA Polymerase and three other commercially available high-fidelity enzymes.

PolymeraseTotal extension step time per cycle for a 4-kb product
PrimeSTAR Max polymerase 10 sec minimum*
Company T polymerase 120 sec
Company N polymerase 120 sec
Company I polymerase 240 sec


*See data in Figure 1 for an example of amplification of a 4-kb product with a 10 sec extension step. Routine recommendations: when template quantity is 200 ng/50 µl or less, an extension step of 5 sec/kb is recommended. When template quantity exceeds 200 ng/50 µl, an extension step of 30–60 sec/kb is recommended.


Conclusions  

PrimeSTAR Max DNA Polymerase offers the highest fidelity and fastest extension speed of any commercially available polymerase. It was shown to provide superior yields for a series of amplification products up to 4 kb in length when compared to polymerases from other manufacturers. PrimeSTAR Max DNA Polymerase also requires a far shorter extension time than other commercially available high-fidelity polymerases, making it a better choice for high-fidelity PCR performed under fast cycling conditions.

Related products

Cat. # Product Size License Quantity Details
R045A PrimeSTAR® Max DNA Polymerase 100 Rxns USD $227.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data

The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by  analysis of sequencing data
The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data. Eight arbitrarily selected GC-rich regions were amplified with PrimeSTAR Max DNA Polymerase or other DNA polymerases, using Thermus thermophilus HB8 genomic DNA as template. PCR products (~500 bp each) were each cloned into a suitable plasmid. Multiple clones were selected per respective amplification product and were subjected to sequence analysis. Sequence analysis of DNA fragments amplified using PrimeSTAR Max DNA Polymerase demonstrated only 9 mismatched bases per 230,129 total bases. This is higher fidelity than an alternative high-fidelity enzyme from Company A, and 10-fold higher fidelity than Taq DNA polymerase.

Back

R045A: PrimeSTAR Max DNA Polymerase

R045A: PrimeSTAR Max DNA Polymerase
R045B PrimeSTAR® Max DNA Polymerase 400 Rxns USD $761.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.  Cat. # R045B contains 4 of Cat. # R045A. Please refer to Cat. # R045A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data

The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by  analysis of sequencing data
The mutation frequency of PrimeSTAR Max DNA Polymerase and four other DNA Polymerases was examined by analysis of sequencing data. Eight arbitrarily selected GC-rich regions were amplified with PrimeSTAR Max DNA Polymerase or other DNA polymerases, using Thermus thermophilus HB8 genomic DNA as template. PCR products (~500 bp each) were each cloned into a suitable plasmid. Multiple clones were selected per respective amplification product and were subjected to sequence analysis. Sequence analysis of DNA fragments amplified using PrimeSTAR Max DNA Polymerase demonstrated only 9 mismatched bases per 230,129 total bases. This is higher fidelity than an alternative high-fidelity enzyme from Company A, and 10-fold higher fidelity than Taq DNA polymerase.

Back

R045B: PrimeSTAR Max DNA Polymerase

R045B: PrimeSTAR Max DNA Polymerase


Capturem his-tagged purification

Simplified purification of active, secreted his-tagged proteins

Purify active, secreted his-tagged proteins from cell-culture supernatants in 15 minutes.

Tech note: Purification of active, secreted proteins


Tech note

A revolutionary solution for recombinant protein purification

Learn about a novel solution for recombinant protein purification from mammalian and bacterial cell lysates.

Capturem technology overview


PCR product

Find high-quality solutions for applications downstream of your PCR

Looking for quick and dependable purification of your amplicon? Easily clean up your PCR product, either from an agarose gel or directly from the reaction.

Plan on using your amplicon for cloning? Seamlessly insert your PCR product into any vector, at any site of linearization using ligation-independent cloning.

Clean it Clone it

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  • PCR master mixes
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  • In-Fusion seamless cloning
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