In-Fusion molar ratio calculator
Calculating the optimal amounts of vector and insert to use in the In-Fusion Cloning reaction is easy with our In-Fusion molar ratio calculator—simply provide the size of your insert and vector in base pairs below.
New!
The In-Fusion molar ratio calculator is now optimized for multi-insert cloning. Simply choose the number of inserts (up to 5).
Notes:
- The molar ratio of insert:vector is set to 2:1, as recommend by our In-Fusion Cloning protocol. The molar ratio stays the same with multiple inserts. For example, the molar ratio of two inserts with one vector should be 2:2:1.
- The total DNA amount (insert + vector) provided by the calculator is 200 ng, which is optimal for a 10-µl In-Fusion Cloning reaction.
- Seamless cloning is not recommended for inserts less than 50 nucleotides.
Cloning shouldn't be work, it should just work ...
With In-Fusion technology, you will be able to perform directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction, with >95% cloning efficiency. Our In-Fusion bundles are complete systems that include all of the reagents you need for your cloning experiments: PCR enzyme, competent cells, and a kit for PCR cleanup or gel extraction. We have optimized the reagents to work together to give you the right clone the first time.
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