Traditional molecular cloning
Restriction enzyme overview
- General information about restriction enzymes
- Star activity of restriction enzymes
- Inactivation of restriction enzymes
- Buffer activity with restriction enzymes
- Universal buffers for double digestion with restriction enzymes
- Restriction enzymes affected by methylation
- Methylation-sensitive restriction enzymes
- QC of restriction enzymes
- Ligation cloning overview
- Ligation product guide
Methylation-sensitive restriction enzymes (MSREs)
Methylation-Sensitive Restriction Enzymes (MSREs) play a role in the analysis of methylated DNA, as they are used to analyze the methylation status of cytosine residues in CpG sequences. These restriction enzymes, as their name implies, are not able to cleave methylated-cytosine residues, leaving methylated DNA intact. MSREs cleave DNA at specific unmethylated-cytosine residues and DNA is then amplified by PCR following digestion. Because unmethylated DNA is digested by MSREs, the only amplification products detected are methylated DNA. It is important to fully digest the DNA before PCR amplification to generate accurate data.
The table below lists MSREs that may be useful for mammalian epigenetics analyses when used with CpG methylase.
|Restriction Enzyme||Recognition Sequence|
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