DNA ligation products: selection guide
The selection of a DNA ligation kit requires consideration of several factors. During cloning projects it is helpful to assess whether the ligation involves cloning a long insert, whether rapid ligation would aid the overall workflow, and whether the type of ends being ligated are blunt, A-overhang (TA-cloning), or sticky (cohesive).
Depending on experimental conditions, transformation efficiency can sometimes be improved by limiting ligation reaction volume and thereby using a smaller volume of ligation mix to transform cells. High-throughput cloning studies may require streamlined ligation reaction protocol approaches to save time and minimize sample handling steps. We offer a range of DNA ligation kit options for each of these scenarios, so use the following guide to help choose the ligation kit that is best for your experiment.
| Ligation Reaction Type | |||
|---|---|---|---|
| Fast Ligations | Long Inserts & BAC Library Construction | Small Volume Reactions | High-Throughput |
| Just add DNA & Ligate in 5 min. with... DNA Ligation Kit, Mighty Mix |
Ligate inserts up to 10 kb with ... DNA Kit LONG |
Premix for Low Reaction Volume with ... DNA Ligation Kit, Ver. 2.1 |
Just add DNA & Ligate in 5 min. with... DNA Ligation Kit, Mighty Mix |
| Ligate in 3 min. with... DNA Ligation Kit, Ver. 2.1 |
Just add DNA & Ligate in 5 min. with ... DNA Ligation Kit, Mighty Mix |
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