- In-Fusion Cloning
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DNA Ligation Kit, Mighty Mix
The DNA Ligation Kit, Mighty Mix is a 2X ligation premix for high-efficiency ligations, especially for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The 2X ligation mix is compatible with small ligation reaction volumes (10 µl) for when DNA quantity is a limiting factor, and it contains sufficient reagent for 75–150 reactions. The reaction mixtures from this DNA ligation kit can be used directly in bacterial transformation.
- Enables high-efficiency ligations for cohesive, blunt-end, and TA cloning
- 2X ligation mix adds convenience and eliminates extra pipetting and purification steps
- Ligation reactions can be quickly performed at either 5 or 30 minutes depending on DNA type
- Reaction volumes are scalable
- Highly efficient and fast DNA ligation
- Small reaction volume ligation
- TA and blunt-end cloning
Storage and use
This kit is stable through 50 freeze-thaw cycles. The 2X ligation mix should be thawed on ice (5–10 minutes) and mixed well by pipette prior to use.
Hayashi, K., Nakazawa, M., Ishizaki, Y., Hiraoka, N. & Obayashi, A. Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–31 (1986).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab in the product table.
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