Poly(A) Polymerase catalyzes the incorporation of adenine residues into the 3' termini of RNA, effectively adding a poly(A) tail to RNA. The enzyme uses single-stranded RNA as a primer during poly(A) tailing. Poly(A) Polymerase is supplied in a buffer of 25 mM Tris-HCl (pH 7.9), 500 mM NaCl, 1 mM EDTA, 0.1 mM DTT, and 50% glycerol.
- Adding a poly(A) tail to RNA
- 3'-end RNA labeling
E. coli B
One unit is defined as the amount of enzyme that incorporates 1 nmol of AMP into tRNA in 10 minutes at 37°C and pH 7.9 using ATP as the substrate.
Gething, M. J., Bye, J., Skehel, J. & Waterfield, M. Cloning and DNA sequence of double-stranded copies of haemagglutinin genes from H2 and H3 strains elucidates antigenic shift and drift in human influenza virus. Nature 287, 301–6 (1980).
Krug, M. S. & Berger, S. L. First-strand cDNA synthesis primed with oligo(dT). Methods Enzymol. 152, 316–25 (1987).
Winter, G. & Brownlee, G. G. 3'End labelling of RNA with 32P suitable for rapid gel sequencing. Nucleic Acids Res. 5, 3129–39 (1978).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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