Mung Bean Nuclease
Mung Bean Nuclease acts as a preferential DNA and RNA endonuclease, hydrolyzing single-stranded DNA and RNA into 5'-phosphate- and 3'-hydroxyl-containing products. If used at very high concentrations, Mung Bean Nuclease is also capable of degrading double-stranded DNA, RNA or DNA/RNA fragments from both ends. Due to its single-stranded DNA and RNA endonuclease activity, Mung Bean Nuclease is often used during transcript mapping, duplex or hybrid DNA and RNA blunt ending, and cDNA preparation.
- Double-stranded DNA, RNA, or DNA/RNA blunt ending
- cDNA hairpin loop cleavage
Mung bean sprouts
Supplied with 10X Reaction Buffer: 300 mM sodium acetate (pH 5.0), 1000 mM NaCl, 10 mM zinc acetate, and 50% glycerol
One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into acid-soluble product in 1 minute at 37°C and pH 5.0.
Kowalski, D., Kroeker, W. D. & Laskowski, M. Mung bean nuclease I. Physical, chemical, and catalytic properties. Biochemistry 15, 4457–63 (1976).
Kroeker, W. D., Kowalski, D. & Laskowski, M. Mung bean nuclease I. Terminally directed hydrolysis of native DNA. Biochemistry 15, 4463–7 (1976).
Mathis, D. J., Elkaim, R., Kédinger, C., Sassone-Corsi, P. & Chambon, P. Specific in vitro initiation of transcription on the adenovirus type 2 early and late EII transcription units. Proc. Natl. Acad. Sci. U. S. A. 78, 7383–7 (1981).
Sheflin, L. G. & Kowalski, D. Altered DNA conformations detected by mung bean nuclease occur in promoter and terminator regions of supercoiled pBR322 DNA. Nucleic Acids Res. 13, 6137–54 (1985).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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