Mung Bean Nuclease

Mung Bean Nuclease acts as a preferential DNA and RNA endonuclease, hydrolyzing single-stranded DNA and RNA into 5'-phosphate- and 3'-hydroxyl-containing products. If used at very high concentrations, Mung Bean Nuclease is also capable of degrading double-stranded DNA, RNA or DNA/RNA fragments from both ends. Due to its single-stranded DNA and RNA endonuclease activity, Mung Bean Nuclease is often used during transcript mapping, duplex or hybrid DNA and RNA blunt ending, and cDNA preparation.

Cat. #	Product	Size	Price
2420B	Mung Bean Nuclease	10,000 Units	USD $388.00
Mung Bean Nuclease digests single-stranded nucleic acids (both DNA and RNA). If used at very high concentrations, it is capable of degrading double-stranded DNA fragments. Mung Bean Nuclease is supplied in 10 mM Tris-HCl (pH 7.5), 0.1 mM zinc acetate and 50% glycerol. Cat. # 2420B contains 5 of Cat. # 2420A. Please refer to Cat. # 2420A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2420B: Mung Bean Nuclease
2420A	Mung Bean Nuclease	2,000 Units	USD $99.00
Mung Bean Nuclease digests single-stranded nucleic acids (both DNA and RNA). If used at very high concentrations, it is capable of degrading double-stranded DNA fragments. Mung Bean Nuclease is supplied in 10 mM Tris-HCl (pH 7.5), 0.1 mM zinc acetate and 50% glycerol. Cat. # 2420B contains 5 of Cat. # 2420A. Please refer to Cat. # 2420A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2420A: Mung Bean Nuclease

More Information

Applications

Double-stranded DNA, RNA, or DNA/RNA blunt ending
cDNA hairpin loop cleavage

Buffer

Supplied with 10X Reaction Buffer: 300 mM sodium acetate (pH 5.0), 1000 mM NaCl, 10 mM zinc acetate, and 50% glycerol

Product citations

Kowalski, D., Kroeker, W. D. & Laskowski, M. Mung bean nuclease I. Physical, chemical, and catalytic properties. Biochemistry 15, 4457–63 (1976).

Kroeker, W. D., Kowalski, D. & Laskowski, M. Mung bean nuclease I. Terminally directed hydrolysis of native DNA. Biochemistry 15, 4463–7 (1976).

Mathis, D. J., Elkaim, R., Kédinger, C., Sassone-Corsi, P. & Chambon, P. Specific in vitro initiation of transcription on the adenovirus type 2 early and late EII transcription units. Proc. Natl. Acad. Sci. U. S. A. 78, 7383–7 (1981).

Sheflin, L. G. & Kowalski, D. Altered DNA conformations detected by mung bean nuclease occur in promoter and terminator regions of supercoiled pBR322 DNA. Nucleic Acids Res. 13, 6137–54 (1985).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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