S1 Nuclease is an endonuclease that specifically degrades single-stranded nucleic acids, including the single-stranded regions of duplex DNA, RNA, or DNA/RNA. S1 Nuclease preferentially targets DNA over RNA. This endonuclease can also introduce single-stranded nicks and breaks in duplex DNA, RNA, and DNA/RNA. S1 Nuclease is supplied in a buffer of 10 mM sodium acetate (pH 4.6), 150 mM NaCl, 0.05 mM ZnSO4 and 50% glycerol.
- Nuclease protection assays
- Double-stranded DNA end blunting
- RNA transcript mapping
- cDNA hairpin removal
Supplied with 10X Reaction Buffer: 300 mM sodium acetate (pH 4.6), 2.8 M NaCl and 10 mM ZnSO4
One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into an acid-soluble product in 1 minute at 37°C and pH 4.6.
Ando, T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim. Biophys. Acta 114, 158–68 (1966).
Berk, A. J. & Sharp, P. A. Spliced early mRNAs of simian virus 40. Proc. Natl. Acad. Sci. U. S. A. 75, 1274–8 (1978).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor laboratory press. New York (1989). doi:574.873224 1/1989
Wiegand, R. C., Godson, G. N. & Radding, C. M. Specificity of the S1 nuclease from Aspergillus oryzae. J. Biol. Chem. 250, 8848–55 (1975).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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