Shrimp alkaline phosphatase (SAP)
Shrimp alkaline phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of 5'-termini DNA and RNA phosphomonoesters, but does not degrade diphosphate or triphosphate linkages. The enzyme acts on 5' protruding, recessive, and blunt 5'-termini. Unlike Calf intestinal alkaline phosphatase, SAP is irreversibly heat inactivated at 65°C for 15 minutes, allowing for streamlining of cloning experiments.
- Dephosphorylation of DNA 5'-termini
- Reduction of vector re-ligation and background
Pichia pastoris that has been genetically modified with a gene coding a shrimp alkaline phosphatase.
Supplied with 1 ml 10X SAP Buffer [500 mM Tris-HCl (pH 9.0), 50 mM MgCl2]
One unit is defined as the amount of enzyme required to produce 1 µmol of p-nitrophenol per minute at 37°C (pH 9.8) using p-nitrophenylphosphate as the substrate.
Olsen, R. L., Overbø, K. & Myrnes, B. Alkaline phophatase from the hepatopancreas of shrimp (Pandalus borealis): A dimeric enzyme with catalytically active subunits. Comp. Biochem. Physiol. Part B Comp. Biochem. 99, 755–761 (1991).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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