Calf intestinal alkaline phosphatase (CIAP)
Calf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5'-phosphate termini from DNA, RNA, dNTPs and rNTPs. This enzyme can be used to reduce linear vector self-ligation and vector background during cloning. CIAP is supplied in a buffer of 10 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2, and 50% glycerol.
More Information
Applications
- Reduction of linear vector self-ligation and vector background during cloning
- Dephosphorylation of DNA prior to kinase labeling reactions
- Template preparation prior to 5'-end labeling
- Protein dephosphorylation
Source
Yeast
Storage
–20°C
Notes
Calf intestinal alkaline phosphatase (CIAP) cannot be completely heat inactivated, and therefore it is recommended that the DNA product be purified by gel or spin column purification or phenol-chloroform extraction.
Buffer
Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].
Unit definition
One unit is defined as the amount of enzyme that generates 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 37°C and pH 9.8.
Concentration
10–30 U/µl
Product citations
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning : a laboratory manual. (Cold Spring Harbor Laboratory, 1989).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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