Ribonuclease H (RNase H)
Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation. Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol.
- Removal of RNA template following second-strand cDNA synthesis
- Removal of mRNA poly(A) sequence
- Reverse transcription
Recombinant E. coli
One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.
Schultz, S. J. & Champoux, J. J. RNase H activity: structure, specificity, and function in reverse transcription. Virus Res. 134, 86–103 (2008).
Vournakis, J. N., Efstratiadis, A. & Kafatos, F. C. Electrophoretic patterns of deadenylylated chorion and globin mRNAs. Proc. Natl. Acad. Sci. U. S. A. 72, 2959–63 (1975).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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