Exonuclease III

Exonuclease III is a 3' to 5' exonuclease that removes mononucleotides from the 3'-hydroxyl termini of double-stranded DNA or DNA-RNA hybrids. Because a set number of mononucleotides are generated during each degradation, double-stranded DNA degradation is progressive. Exonuclease III will degrade double-stranded DNA containing a blunt end, a 5'-overhang, or a nick, but it will not degrade DNA with a 3'-overhang containing four or more bases. This 3' to 5' exonuclease can be inactivated by heating it at 65°C for 5 min. Exonuclease III is supplied in a buffer of 25 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, and 50% glycerol.

Cat. #	Product	Size	Price
2170B	Exonuclease III	25,000 Units	USD $259.00
Exonuclease III is a 3'→5' exonuclease that is specific for double-stranded DNA or DNA-RNA hybrids. It will degrade double-stranded DNA with a blunt end, a 5'-overhang, or a nick, but it will not degrade DNA with a 3'-overhang of at least 4 bases. Exonuclease III is supplied in 25 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT and 50% glycerol. Cat. # 2170B contains 5 of Cat. # 2170A. Please refer to Cat. # 2170A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2170B: Exonuclease III
2170A	Exonuclease III	5,000 Units	USD $67.00
Exonuclease III is a 3'→5' exonuclease that is specific for double-stranded DNA or DNA-RNA hybrids. It will degrade double-stranded DNA with a blunt end, a 5'-overhang, or a nick, but it will not degrade DNA with a 3'-overhang of at least 4 bases. Exonuclease III is supplied in 25 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT and 50% glycerol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2170A: Exonuclease III

More Information

Applications

Preparation of single-stranded DNA for dideoxy sequencing
Production of nested deletions in double-stranded DNA
DNA-protein interaction analysis
Site-directed mutagenesis

Buffer

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 8.0), 50 mM MgCl₂, 100 mM DTT]

Product citations

Guo, L.H. & Wu, R. [4] Exonuclease III: Use for DNA sequence analysis and in specific deletions of nucleotides. Methods Enzymol. 100, 60–96 (1983).

Wu, C. An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts. Nature 317, 84–87 (1985).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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