DNA Polymerase I, Klenow Fragment
DNA Polymerase I, Klenow Fragment (Large Fragment) is a proteolytic product of E. coli DNA Polymerase I, which possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks the 5'→3' exonuclease activity of full-length DNA Polymerase I. The lack of 5'→3' exonuclease activity enables the DNA Polymerase I, Klenow Fragment to be used in random primer labeling and DNA sequencing experiments in addition to second-strand cDNA synthesis. The enzyme is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mmDTT and 50% glyerol (2140A/B) or ethylene glycol (2140AK/BK).
- Random primer labeling
- Second-strand cDNA synthesis
- Dideoxy DNA sequencing
- 2140AK/BK are sequencing grade reagents
Recombinant E. coli
One unit is defined as the amount of enzyme required to incorporate 10 nmol of total nucleotides into acid-insoluble product in 30 minutes at 37°C and pH 7.4 using poly d(A-T) as the template-primer.
Supplied with 10X Reaction Buffer [100 mM Tris-HCl (pH 7.5), 70 mM MgCl2 and 1 mM DTT].
2140A/B: 2–5 U/µl
2140AK/BK: 2 U/µl
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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