BAL 31 Nuclease
BAL 31 Nuclease acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'-phosphate and 3'-hydroxyl termini. This enzyme also possesses single-stranded DNA and RNA endonuclease activity and is capable of cleaving at DNA/RNA nicks and gaps. It requires Ca2+ as an essential cofactor for its exonuclease and endonuclease activities.
BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl2, 5 mM MgCl2, 1 mM EDTA and 50% glycerol.
- Progressive deletion of DNA and RNA fragments
Alteromonas espejiana BAL 31
Supplied with 2X Reaction Buffer [40 mM Tris-HCl (pH 8.0), 1,200 mM NaCl, 24 mM MgCl2, 24 mM CaCl2, and 2 mM EDTA].
One unit is defined as the amount of enzyme that produces 1 µg of acid-soluble products from heat-denatured calf thymus DNA in 1 minute at 30°C and pH 8.0.
Gray, H. B. et al. The extracellular nuclease from Alteromonas espejiana: an enzyme highly specific for nonduplex structure in nominally duplex DNA. Gene Amplif. Anal. 2, 169–203 (1981).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning : a laboratory manual. (Cold Spring Harbor Laboratory, 1989).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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