BAL 31 Nuclease

BAL 31 Nuclease acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'-phosphate and 3'-hydroxyl termini. This enzyme also possesses single-stranded DNA and RNA endonuclease activity and is capable of cleaving at DNA/RNA nicks and gaps. It requires Ca²⁺ as an essential cofactor for its exonuclease and endonuclease activities.

BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl₂, 5 mM MgCl₂, 1 mM EDTA and 50% glycerol.

Cat. #	Product	Size	Price
2510B	BAL 31 Nuclease	250 Units	USD $452.00
BAL 31 Nuclease has two types of activity. It acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'- and 3'-ends. It also possesses single-strand endonuclease activity. BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl₂, 5 mM MgCl₂, 1 mM EDTA and 50% glycerol. CCat. # 2510B contains 5 of Cat. # 2510A. Please refer to Cat. # 2510A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2510B: BAL 31 Nuclease
2510A	BAL 31 Nuclease	50 Units	USD $116.00
BAL 31 Nuclease has two types of activity. It acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'- and 3'-ends. It also possesses single-strand endonuclease activity. BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl₂, 5 mM MgCl₂, 1 mM EDTA and 50% glycerol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2510A: BAL 31 Nuclease

More Information

Applications

Progressive deletion of DNA and RNA fragments

Buffer

Supplied with 2X Reaction Buffer [40 mM Tris-HCl (pH 8.0), 1,200 mM NaCl, 24 mM MgCl₂, 24 mM CaCl_2, and 2 mM EDTA].

Product citations

Gray, H. B. et al. The extracellular nuclease from Alteromonas espejiana: an enzyme highly specific for nonduplex structure in nominally duplex DNA. Gene Amplif. Anal. 2, 169–203 (1981).

Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular cloning : a laboratory manual. (Cold Spring Harbor Laboratory, 1989).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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