Terminal Deoxynucleotidyl Transferase (TdT)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the incorporation of single deoxynucleotides into the 3'-OH terminus of single- or double-stranded DNA, making it an effective agent in 3' end labeling applications such as Terminal dUTP nick end labeling (TUNEL). Terminal Deoxynucleotidyl Transferase (TdT) requires an oligodeoxynucleotide of at least three bases as a primer, but is not template-dependent.
- Okayama-Berg methods involving complementary homopolymer tailing of vectors and cDNA
- 3'-end labeling of DNA Fragments using [32P]dNTP or [32P]ddNTP
- 5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl2, 0.5 mM DTT]
- 0.1% BSA
Recombinant E. coli
Endonuclease, exonuclease and nickase activities were not detected via gel electrophoresis.
One unit is the amount of enzyme that incorporates 1 nmol of [3H]dATP into acid-insoluble product in 1 hour at 37°C and pH 7.2 using activated, heat-denatured and DNase-treated calf thymus DNA as the initiator.
60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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