Terminal Deoxynucleotidyl Transferase (TdT)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the incorporation of single deoxynucleotides into the 3'-OH terminus of single- or double-stranded DNA, making it an effective agent in 3' end labeling applications such as Terminal dUTP nick end labeling (TUNEL). Terminal Deoxynucleotidyl Transferase (TdT) requires an oligodeoxynucleotide of at least three bases as a primer, but is not template-dependent.
- Okayama-Berg methods involving complementary homopolymer tailing of vectors and cDNA
- 3'-end labeling of DNA Fragments using [32P]dNTP or [32P]ddNTP
- 5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl2, 0.5 mM DTT]
- 0.1% BSA
Endonuclease, exonuclease and nickase activities were not detected via gel electrophoresis.
60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2021 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.