Terminal Deoxynucleotidyl Transferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the incorporation of single deoxynucleotides into the 3'-OH terminus of single- or double-stranded DNA, making it an effective agent in 3' end labeling applications such as Terminal dUTP nick end labeling (TUNEL). Terminal Deoxynucleotidyl Transferase (TdT) requires an oligodeoxynucleotide of at least three bases as a primer, but is not template-dependent.

Cat. #	Product	Size	Price
2230B	Terminal Deoxynucleotidyl Transferase	1,500 Units	USD $246.00
Terminal Deoxynucleotidyl Transferase (TdT) does not require a template for activity, and catalyzes the incorporation of deoxynucleotides into the 3'-OH termini of single- or double-stranded DNA. It requires an oligodeoxynucleotide of at least three bases as a primer. Cat. # 2230B contains 5 of Cat. # 2230A. Please refer to Cat. # 2230A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2230B: Terminal Deoxynucleotidyl Transferase
2230A	Terminal Deoxynucleotidyl Transferase	300 Units	USD $64.00
Terminal Deoxynucleotidyl Transferase (TdT) does not require a template for activity, and catalyzes the incorporation of deoxynucleotides into the 3'-OH termini of single- or double-stranded DNA. It requires an oligodeoxynucleotide of at least three bases as a primer. Cat. #2230B contains 5.0 of Cat. #2230A. Please refer to Cat. #2230A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2230A: Terminal Deoxynucleotidyl Transferase

More Information

Applications

Okayama-Berg methods involving complementary homopolymer tailing of vectors and cDNA
3'-end labeling of DNA Fragments using [³²P]dNTP or [³²P]ddNTP
TUNEL

Supplied buffer

5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl₂, 0.5 mM DTT]
0.1% BSA

Purity

Endonuclease, exonuclease and nickase activities were not detected via gel electrophoresis.

Form

60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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