DNase I (RNase-free)

Applications

• Template DNA digestion following in vitro transcription
• Genomic DNA digestion prior to RT-PCR
• Nick translation with DNA polymerase
• Shotgun DNA library construction
• Footprinting analysis

Components

Supplied Buffer (10X)

Source

Recombinant non-animal host containing bovine pancreas DNase I-encoding plasmid

Storage

–20°C

Unit definition

One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 25°C (pH 5.0) using calf thymus DNA as the substrate (Kunitz unit).

Concentration

5 U/µl

Properties

Recombinant DNase I (RNase-free) is heat inactivated by incubating at 80°C for 10 minutes.

References

Anderson, S. Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Res. 9, 3015–27 (1981).   

Galas, D. J. & Schmitz, A. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res. 5, 3157–70 (1978).

Green, M. R., Maniatis, T. & Melton, D. A. Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell 32, 681–94 (1983).

Kunitz, M. Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity. J. Gen. Physiol. 33, 349–62 (1950).

Rigby, P. W., Dieckmann, M., Rhodes, C. & Berg, P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113, 237–51 (1977).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.