DNase I (RNase-free)

DNase I (RNase-free) is a recombinant endonuclease that nonspecifically catalyzes the degradation of both single- and double-stranded DNA and DNA-RNA hybrids, producing 5'-phosphate and 3'-hydroxyl termini-containing oligonucleotides of varying lengths. The protease activity of DNase I endonuclease has been eliminated; therefore, this enzyme is stable at its optimal (neutral) pH range and is suitable for RNA preparation at neutral pH.

Cat. #	Product	Size	Price
2270B	Recombinant DNase I (RNase-free)	5,000 Units	USD $444.00
Recombinant DNase I (RNase-free) is an endonuclease that catalyzes, to the same degree, the random degradation of both single- and double-stranded DNA and produces 5'-P terminal oligonucleotides. This enzyme does not exhibit protease activity. This enzyme is stable around its optimum neutral pH range and is suitable for RNA preparation at neutral pH. Cat. # 2270B contains 5 of Cat. # 2270A. Please refer to Cat. # 2270A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2270B: Recombinant DNase I (RNase-free)
2270A	Recombinant DNase I (RNase-free)	1,000 Units	USD $112.00
Recombinant DNase I (RNase-free) is an endonuclease that catalyzes, to the same degree, the random degradation of both single- and double-stranded DNA and produces 5'-P terminal oligonucleotides. This enzyme does not exhibit protease activity. This enzyme is stable around its optimum neutral pH range and is suitable for RNA preparation at neutral pH. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2270A: Recombinant DNase I (RNase-free)

More Information

Applications

Template DNA digestion following in vitro transcription
Genomic DNA digestion prior to RT-PCR
Nick translation with DNA polymerase
Shotgun DNA library construction
Footprinting analysis

Components

Supplied Buffer (10X)

Tris-HCl, pH7.5	400 mM
MgCl₂	80 mM
DTT	50 mM

Source

Recombinant non-animal host containing bovine pancreas DNase I-encoding plasmid

Storage

–20°C

Unit definition

One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 25°C (pH 5.0) using calf thymus DNA as the substrate (Kunitz unit).

Concentration

5 U/µl

Properties

Recombinant DNase I (RNase-free) is heat inactivated by incubating at 80°C for 10 minutes.

References

Anderson, S. Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Res. 9, 3015–27 (1981).

Galas, D. J. & Schmitz, A. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res. 5, 3157–70 (1978).

Green, M. R., Maniatis, T. & Melton, D. A. Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell 32, 681–94 (1983).

Kunitz, M. Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity. J. Gen. Physiol. 33, 349–62 (1950).

Rigby, P. W., Dieckmann, M., Rhodes, C. & Berg, P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113, 237–51 (1977).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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