Micrococcal Nuclease
Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA or RNA, especially at AT- or AU-rich regions. The enzyme will also digest double-stranded DNA or RNA, making it an essential component of chromatin immunoprecipitation (ChIP) assays. Micrococcal Nuclease digests 5'-phosphodiester bonds of DNA and RNA, yielding 3'-phosphate mononucleotides and oligonucleotides. This enzyme requires Ca2+ as a cofactor for its activity and is completely inactivitated by EDTA or EGTA.
More Information
Applications
- Digestion of nucleotides included in crude cell extract
- Digestion of chromatin for nucleosome preparation
- Chromatin immunoprecipitation (ChIP)
Supplied buffer
10X Micrococcal Nuclease [200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl2]
Form
50 mM Tris-HCl (pH 8.0), 10 mM 2-mercaptoethanol, 50 mM NaCl, 50% glycerol
Product citations
Lefevre, P. & Bonifer, C. Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease. Methods Mol. Biol. 325:315–325 (2006).
Heins, J.N. et al. Procedures Nucl. Acids Res. 1:79 (1966).
Pelham, H.R. & Jackson, R.J. An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates. Eur. J. Biochem. 67:247–256 (1976).
Telford, D.J. & Stewart, B.W. Micrococcal nuclease: Its specificity and use for chromatin analysis. Int. J. Biochem. 21:127–137 (1989).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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