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  • ‹ Back to Ligation kits
  • DNA ligation kits, quick
  • DNA ligation kit, long fragments
  • DNA Blunting Kit
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Home › Products › Cloning › Ligation kits › DNA Blunting Kit

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DNA Blunting Kit

DNA Blunting Kit for converting 3' and 5' overhangs to blunt ends.

The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3'→5' exonuclease and 5'→3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can then be ligated into a vector using the same optimized buffer system employed in our: DNA Ligation Kit, Mighty Mix; TaKaRa DNA Ligation Kit LONG; and Quick DNA ligation kit products. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without the need for further DNA purification.

Cat. # Product Size Price License Quantity Details
6025 DNA Blunting Kit 20 Rxns $220.00

The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt ends. This conversion is accomplished by the 3'→5' exonuclease and 5'→3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can be ligated efficiently into a vector using the same optimized buffer system used in Takara's DNA Ligation Kits, then used directly in bacterial transformation or in vitro packaging procedures without DNA purification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Resources

More Information

Applications

  • Quick and easy blunting of DNA fragment ends

Product citations

Sambrook, J., Fritsch, E. F. & Maniatis, T. in Mol. Cloning A Lab. Manual. Cold Spring Harb. Lab. Press 931–957 (Cold Spring Harbor Laboratory Press, 1989).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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