- In-Fusion Cloning
- Competent cells
- Ligation kits
- Mutagenesis kits
- Ligation enzymes
- Restriction enzymes
- Modifying enzymes
- Legacy cloning products
- X-Gal and IPTG
- Linkers, primers, and cloning vectors
- Agarose gel electrophoresis
- Nucleic acid extraction
DNA Blunting Kit
The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3'→5' exonuclease and 5'→3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can then be ligated into a vector using the same optimized buffer system employed in our: DNA Ligation Kit, Mighty Mix; TaKaRa DNA Ligation Kit LONG; and Quick DNA ligation kit products. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without the need for further DNA purification.
- Quick and easy blunting of DNA fragment ends
Sambrook, J., Fritsch, E. F. & Maniatis, T. in Mol. Cloning A Lab. Manual. Cold Spring Harb. Lab. Press 931–957 (Cold Spring Harbor Laboratory Press, 1989).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.