pRI 201 DNA transformation vectors

The Ri Plasmid pRI 201 is designed for the transformation and expression of target genes from plant cells. This series of binary vectors for plant transformation retains the backbone of the pRI 101 vectors, including an alcohol dehydrogenase (ADH) gene-derived 5'-untranslated region (5'-UTR) downstream of the cauliflower mosaic virus (CaMV promoter)-derived 35S promoter. Additionally, these vectors have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI 101 vector series.

The Ri Plasmid pRI 201 is designed for the transformation and expression of target genes from plant cells. This series of binary vectors for plant transformation retains the backbone of the pRI 101 vectors, including an alcohol dehydrogenase (ADH) gene-derived 5'-untranslated region (5'-UTR) downstream of the cauliflower mosaic virus (CaMV promoter)-derived 35S promoter. Additionally, these vectors have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI101 vector series.

Multigene transformation with a single vector can be achieved by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the vector's second multiple cloning site (MCS2) located downstream of the HSP terminator.

We offer two types of the pRI 201 DNA vectors, pRI 201-AN DNA and pRI 201-ON DNA. pRI 201-AN DNA contains an Arabidopsis ADH-derived 5'-UTR (AtADH 5'-UTR) and is suitable for dicotyledonous plant transformation. pRI 201-ON DNA contains a rice ADH-derived 5'-UTR (OsADH 5'-UTR) and is suitable for monocotyledonous plant transformation. The pRI 201-AN DNA and pRI 201-ON DNA binary vectors for plant transformation have a mutant-type replication origin (Ri ori) from the Rhizobium rhizogenes Ri plasmid. These vectors also contain a replication origin (ColE1 ori) derived from the pUC plasmid; the ColE1 ori allows high copy number replication in E. coli. The vectors' multiple cloning sites, located near the right border (RB) of T-DNA, allows stable target gene integration into the plant chromosome.

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Cat. #	Product	Size
3265	pRI 201-ON DNA	10 ug	USD $455.00
Use pRI 201-AN DNA to transform dicotyledonous plants. Use pRI 201-ON DNA to transform monocotyledonous plants. The presence of 2 multicloning sites enable multigene transformation with a single vector. Vectors contain a replication origin (ColE1 ori) derived from pUC plasmids, which allows maintenance at a high-copy- number in E. coli. pRI 201 DNA series are binary vectors designed for expressing target genes in transformed plant cells. This series of vectors retain the backbone of pRI 101 vectors (Cat.# 3262/3263), which includes an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5' -UTR) (translational enhancer region) downstream of the 35S promoter from cauliflower mosaic virus (CaMV). In addition, they have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI 101 series 2. Further, multigene transformation with a single vector is possible by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the cloning site (MCS2) downstream of the HSP terminator. The pRI 201 series has two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. Carrying an Arabidopsis ADH-derived 5' -UTR (AtADH 5' -UTR), pRI 201-AN DNA is suitable for dicotyledonous plants. With a rice ADH-derived 5' -UTR (OsADH 5' -UTR), pRI 201-ON DNA is compatible with monocotyledonous plants. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3265: pRI 201-ON DNA
3264	pRI 201-AN DNA	10 ug	USD $455.00
Use pRI 201-AN DNA to transform dicotyledonous plants. Use pRI 201-ON DNA to transform monocotyledonous plants. The presence of 2 multicloning sites enable multigene transformation with a single vector. Vectors contain a replication origin (ColE1 ori) derived from pUC plasmids, which allows maintenance at a high-copy- number in E. coli. pRI 201 DNA series are binary vectors designed for expressing target genes in transformed plant cells. This series of vectors retain the backbone of pRI 101 vectors (Cat.# 3262/3263), which includes an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5' -UTR) (translational enhancer region) downstream of the 35S promoter from cauliflower mosaic virus (CaMV). In addition, they have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI 101 series 2. Further, multigene transformation with a single vector is possible by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the cloning site (MCS2) downstream of the HSP terminator. The pRI 201 series has two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. Carrying an Arabidopsis ADH-derived 5' -UTR (AtADH 5' -UTR), pRI 201-AN DNA is suitable for dicotyledonous plants. With a rice ADH-derived 5' -UTR (OsADH 5' -UTR), pRI 201-ON DNA is compatible with monocotyledonous plants. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3264: pRI 201-AN DNA

Overview

Use pRI 201-AN DNA to transform dicotyledonous plant species
Use pRI 201-ON DNA to transform monocotyledonous plant species
Integrate multiple transgenes using a single vector
High copy number (ColE1 ori) replication in E. coli

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Storage

These vectors should be stored at –20°C and used within 2 years of receipt.

Product citations

Nagaya, S., Kawamura, K., Shinmyo, A. & Kato, K. The HSP terminator of Arabidopsis thaliana increases gene expression in plant cells. Plant Cell Physiol. 51, 328–32 (2010).

Nishiguchi, R., Takanami, M. & Oka, A. Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA 4b. MGG Mol. Gen. Genet. 206, 1–8 (1987).

Satoh, J., Kato, K. & Shinmyo, A. The 5'-untranslated region of the tobacco alcohol dehydrogenase gene functions as an effective translational enhancer in plant. J. Biosci. Bioeng. 98, 1–8 (2004).

Sugio, T. et al. Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells. J. Biosci. Bioeng. 109, 170–3 (2010).

Sugio, T., Satoh, J., Matsuura, H., Shinmyo, A. & Kato, K. The 5'-untranslated region of the Oryza sativa alcohol dehydrogenase gene functions as a translational enhancer in monocotyledonous plant cells. J. Biosci. Bioeng. 105, 300–2 (2008).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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