Random Primers are 6-mer and 9-mer deoxyribonucleotide mixtures composed of a random sequence (up to 46 or 49 different sequences, respectively) and a phosphorylated 5'-end. Both the pd(N)6 Random Primer and the pd(N)9 Random Primer contain approximately 150 µg (5 OD) and are suitable for cDNA synthesis and hybridization applications.
- Single-tube cDNA synthesis
- Hybridization experiments using labeled probe (120 pmol primer/µg DNA recommended)
- First-strand cDNA synthesis during RT-PCR
Lyophilized product ships at room temperature. Store at –20°C.
Dissolve pd(N)9 in 1 ml of RNase-Free dH2O (final conc. 50 µM) prior to use in RT-PCR experiments.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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