M13 DNA sequencing primers
M13 DNA sequencing primers enable sequencing inserts in M13mp and pUC vectors. M13 Primer M3 and M13 Primer M4 are forward DNA sequencing primers while M13 Primer RV is a reverse DNA sequencing primer.
- M13 Primer M3: d(GTAAA ACGAC GGCCA GT)
- M13 Primer M4: d(GTTTT CCCAG TCACG AC)
- M13 Primer RV: d(CAGGA AACAG CTATG AC)
- Confirmed by HPLC
- Primer activity confirmed by Sanger dideoxy sequencing method
Huang, Y. F., Chen, S. C., Chiang, Y. S., Chen, T. H. & Chiu, K. P. Palindromic sequence impedes sequencing-by-ligation mechanism. BMC Syst. Biol. 6 Suppl 2, S10 (2012).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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