pNotI phosphorylated linker
The pNotI phosphorylated linker is a cloning reagent containing a phosphate group at its 5'-end, which enables it to be used directly in linker ligation during cloning experiments. The in-frame cloning of a target gene into a vector can be easily performed by using a phosphorylated linker that results in the desired reading frame.
This lyophilized product is a single-stranded oligonucleotide. For use in downstream applications, dissolve in a buffer such as TE, incubate at 70°C for 10 minutes, and anneal by cooling slowly.
Linker, pNotI (10 mer): pd(AGCGGCCGCT)
- Confirmed by HPLC
- Biological activity confirmed by self-ligation and restriction enzyme cleavage
Brown, N. L. & Smith, M. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I. FEBS Lett. 65, 284-7 (1976).
Roberts, R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13 Suppl, r165-200 (1985).
Smith, D. I., Blattner, F. R. & Davies, J. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii. Nucleic Acids Res. 3, 343-53 (1976).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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