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  • ‹ Back to Cloning vectors
  • E. coli-yeast shuttle vectors
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Home › Products › Cloning › Linkers, primers, and cloning vectors › Cloning vectors › DNA cloning vectors

Linkers, primers, and cloning vectors

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DNA cloning vectors

Our DNA cloning vectors may be used for a variety of cloning reactions, such as target gene cloning and expression, site-directed mutagenesis, and expression of genes that can be toxic to host cells. The suite of cloning vectors includes pKF18k-2, pSTV28, pSTV29, pTV118N, pTWV228, and pUC118.

Cat. # Product Size License Quantity Details
3332 pSTV 29 DNA 25 ug USD $73.00

pSTV 29 DNA is a plasmid vector reconstructed from the β-galactosidase gene, with the replication origin of pACYC184 (the chloramphenicol resistance gene for Tn9). There is a pUC119 multicloning site but XbaI and AccI sites are not available. Since this DNA has lower copy numbers relative to pUC-type vectors, it is useful for cloning genes which do harm to the host if expressed in large amounts. Moreover, since this DNA contains a pACYC184 ori, it can be present into the same cell with plasmid vectors such as pUC and pBR.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3332: pSTV29 DNA

3332: pSTV29 DNA
3331 pSTV 28 DNA 25 ug USD $73.00

pSTV 28 DNA is a plasmid vector reconstructed from the β-galactosidase gene, with the replication origin of pACYC184 (the chloramphenicol resistance gene for Tn9). There is a pUC118 multicloning site but XbaI and AccI sites are not available. Since this DNA has lower copy numbers relative to pUC-type vectors, it is useful for cloning genes which do harm to the host if expressed in large amounts. Moreover, since this DNA contains a pACYC184 ori, it can be present into the same cell with plasmid vectors such as pUC and pBR.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3331: pSTV28 DNA

3331: pSTV28 DNA
3328 pTV 118N DNA (0.5 OD) 25 ug USD $73.00

pTV118N DNA contains the sequence CCATGC, which is the NcoI cleavage site and contains the initiation codon (ATG) of lacZ. When a target gene is inserted into the NcoI site, induction and expression of the gene will be under control of the lac promoter and SD sequence of lacZ. This vector is designed to have a distance of 8 bases between the SD sequence of lacZ and the starting codon, in order to improve the efficiency of translational initiation. The translation frame can be confirmed by sequencing the starting codon region using M13 primer RV-N after isolation of the single-strand DNA using helper phage. Note: It is not possible to sequence the single-stranded DNA using a typical M13 forward primer, because the M13 IG region for lacZ in pTV118N is reverse-oriented relative to pUC118.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3328: pTV 118N DNA (0.5 OD)

3328: pTV 118N DNA (0.5 OD)
3324 pUC 118 Hind III/BAP 5 ug USD $71.00

This cloning vector was prepared by cleavage with HindIII (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3324: pUC 118 Hind III/BAP

3324: pUC 118 Hind III/BAP
3323 pUC 118 Pst I/BAP 5 ug USD $71.00

This cloning vector was prepared by cleavage with PstI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3323: pUC118 Pst I/BAP

3323: pUC118 Pst I/BAP
3322 pUC 118 Hinc II/BAP 5 ug USD $71.00

This cloning vector was prepared by cleavage with HincII (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3322: pUC 118 Hinc II/BAP

3322: pUC 118 Hinc II/BAP
3321 pUC 118 BamH I/BAP 5 ug USD $71.00

This cloning vector was prepared by cleavage with BamHI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3321: pUC118 BamH I/BAP

3321: pUC118 BamH I/BAP
3320 pUC 118 EcoR I/BAP 5 ug USD $71.00

This cloning vector was prepared by cleavage with EcoRI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3320: pUC118 EcoR I/BAP

3320: pUC118 EcoR I/BAP
3101 pKF 18k-2 DNA 10 ug USD $73.00

The pKF pUC-derived vector DNA contains two amber stop mutations at the kanamycin resistance gene. When transformed with pKF, supE strains like JM109 but not sup0 strains like MV1184 will grow on kanamycin-containing plates. The pKF DNA may be used for site-directed mutagenesis based on the ODA (Oligonucleotide-directed Dual Amber) method. Using the simplified ODA procedure, a desired mutation can be introduced in only three days. Moreover, pKF DNA is available for target gene cloning and expression via the lac promoter when transformed into supE hosts like JM109. Target genes cloned at the initiation codon (ATG) of the Nde I restriction site (CATATG) have a higher translation efficiency.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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3101: pKF18k-2 DNA

3101: pKF18k-2 DNA

More Information

Vector Information Length (bp)
pKF18k-2 DNA The pKF pUC-derived vectors contain two amber stop mutations at the kanamycin resistance gene. When transformed with pKF, supE strains, like JM109, will grow on kanamycin-containing plates (sup0 strains, such as MV1184, will not). The pKF vectors can be used to perform site-directed mutagenesis based on the Oligonucleotide-directed Dual Amber (ODA) method. Moreover, pKF DNA is available for target gene cloning and expression via the lac promoter when transformed into supE hosts. Target genes cloned at the initiation codon (ATG) of the Nde I restriction site (CATATG) have a higher translation efficiency. 2,204
pSTV28, pSTV29 DNA The pSTV vectors contain a beta-galactosidase gene, a pACYC184 origin of replication, a chloramphenicol resistance gene, and a pUC119 multiple cloning site. Since the pSTV plasmids produce fewer copy numbers compared with pUC-type high copy number plasmids, they are suitable for the expression of genes that may be toxic to host cells. The pSTV28 and pSTV29 vectors contain the pACYC184 origin of replication and can be co-transformed with pUC or pBR vectors. 2,999
pTV118N DNA The pTV118N DNA, a phagemid vector, is constructed from a modified pUC118 plasmid. pTV118N DNA contains the sequence CCATGG, which includes the cleavage sequence for the restriction enzyme NcoI as well as the start codon (ATG) for lacZ. This enables target gene expression at the NcoI site, while protein expression is enabled by the vector's lac promoter. The pTV118N vector also contains a lacZ SD sequence. There are eight bases between the lacZ SD sequence and the initiation codon, allowing high level expression of target genes. Induction of single-stranded DNA by helper phage and its subsequent sequencing with RV-N primer enables accurate sequencing from the start codon site, ensuring an insert's correct translation frame. 3,162
pTWV228 DNA The pTWV228 vector contains a pBR322 origin of replication, an ampicillin-resistance gene, an intergenic region (IG region) of the M13 phage DNA, and a beta-galactosidase gene containing the multiple cloning site of pUC118. This vector is low-copy number, which makes it useful during the expression of genes that present potential toxicity to their host. 4,039
pUC118 DNA The pUC118 vectors can be used to prepare single-stranded DNA. pUC118 DNA was constructed by inserting the intergenic region (IG region) of the M13 phage DNA into a pUC18 plasmid. Co-transformation of pUC118 with the M13K07 helper phage induces the production of single-stranded DNA that is packaged into phage particles and released from bacterial cells. Using this system, large (up to 7 kb) and deletion-free single-stranded DNA can be stably obtained. 3,162


Storage

–20°C

Concentration

250–1,000 µg/ml (except as specifically listed below)

pTV118N: 0.5 µg/µl

pUC118 BAP-treated DNA: 0.1 µg/µl

pKF 18k-2 DNA: 0.5 µg/µl

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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