Home › Products › Cloning › Linkers, primers, and cloning vectors › Cloning vectors › DNA cloning vectors

DNA cloning vectors

Our DNA cloning vectors may be used for a variety of cloning reactions, such as target gene cloning and expression, site-directed mutagenesis, and expression of genes that can be toxic to host cells. The suite of cloning vectors includes pKF18k-2, pSTV28, pSTV29, pTV118N, pTWV228, and pUC118.

Cat. #	Product	Size	Price
3101	pKF 18k-2 DNA	10 ug	$67.00
The pKF pUC-derived vector DNA contains two amber stop mutations at the kanamycin resistance gene. When transformed with pKF, supE strains like JM109 but not sup⁰ strains like MV1184 will grow on kanamycin-containing plates. The pKF DNA may be used for site-directed mutagenesis based on the ODA (Oligonucleotide-directed Dual Amber) method. Using the simplified ODA procedure, a desired mutation can be introduced in only three days. Moreover, pKF DNA is available for target gene cloning and expression via the lac promoter when transformed into supE hosts like JM109. Target genes cloned at the initiation codon (ATG) of the Nde I restriction site (CATATG) have a higher translation efficiency. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
3331	pSTV 28 DNA	25 ug	$67.00
pSTV 28 DNA is a plasmid vector reconstructed from the β-galactosidase gene, with the replication origin of pACYC184 (the chloramphenicol resistance gene for Tn9). There is a pUC118 multicloning site but XbaI and AccI sites are not available. Since this DNA has lower copy numbers relative to pUC-type vectors, it is useful for cloning genes which do harm to the host if expressed in large amounts. Moreover, since this DNA contains a pACYC184 ori, it can be present into the same cell with plasmid vectors such as pUC and pBR. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
3332	pSTV 29 DNA	25 ug	$67.00
pSTV 29 DNA is a plasmid vector reconstructed from the β-galactosidase gene, with the replication origin of pACYC184 (the chloramphenicol resistance gene for Tn9). There is a pUC119 multicloning site but XbaI and AccI sites are not available. Since this DNA has lower copy numbers relative to pUC-type vectors, it is useful for cloning genes which do harm to the host if expressed in large amounts. Moreover, since this DNA contains a pACYC184 ori, it can be present into the same cell with plasmid vectors such as pUC and pBR. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
3328	pTV 118N DNA (0.5 OD)	25 ug	$67.00
pTV118N DNA contains the sequence CCATGC, which is the NcoI cleavage site and contains the initiation codon (ATG) of lacZ. When a target gene is inserted into the NcoI site, induction and expression of the gene will be under control of the lac promoter and SD sequence of lacZ. This vector is designed to have a distance of 8 bases between the SD sequence of lacZ and the starting codon, in order to improve the efficiency of translational initiation. The translation frame can be confirmed by sequencing the starting codon region using M13 primer RV-N after isolation of the single-strand DNA using helper phage. Note: It is not possible to sequence the single-stranded DNA using a typical M13 forward primer, because the M13 IG region for lacZ in pTV118N is reverse-oriented relative to pUC118. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3328: pTV 118N DNA (0.5 OD)
3333	pTWV228 DNA (0.5 OD)	25 ug	$67.00
pTWV228 DNA is a plasmid vector reconstructed from the β-galactosidase gene, with the replication origin of pBR322, an ampicillin-resistance gene, and IG (the intergeneic region of M13 phage DNA). There is a pUC118 multicloning site but the AccI site is not available. Since this vector has lower copy numbers relative to pUC-type vectors, it is useful for cloning genes which do harm to the host if expressed in large amounts. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
3321	pUC 118 BamH I/BAP	5 ug	$67.00
This cloning vector was prepared by cleavage with BamHI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
3320	pUC 118 EcoR I/BAP	5 ug	$65.00
This cloning vector was prepared by cleavage with EcoRI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
3322	pUC 118 Hinc II/BAP	5 ug	$67.00
This cloning vector was prepared by cleavage with HincII (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3322: pUC 118 Hinc II/BAP
3324	pUC 118 Hind III/BAP	5 ug	$67.00
This cloning vector was prepared by cleavage with HindIII (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3324: pUC 118 Hind III/BAP
3323	pUC 118 Pst I/BAP	5 ug	$67.00
This cloning vector was prepared by cleavage with PstI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components

More Information

Vector	Information	Length (bp)
pKF18k-2 DNA	The pKF pUC-derived vectors contain two amber stop mutations at the kanamycin resistance gene. When transformed with pKF, supE strains, like JM109, will grow on kanamycin-containing plates (sup⁰ strains, such as MV1184, will not). The pKF vectors can be used to perform site-directed mutagenesis based on the Oligonucleotide-directed Dual Amber (ODA) method. Moreover, pKF DNA is available for target gene cloning and expression via the lac promoter when transformed into supE hosts. Target genes cloned at the initiation codon (ATG) of the Nde I restriction site (CATATG) have a higher translation efficiency.	2,204
pSTV28, pSTV29 DNA	The pSTV vectors contain a beta-galactosidase gene, a pACYC184 origin of replication, a chloramphenicol resistance gene, and a pUC119 multiple cloning site. Since the pSTV plasmids produce fewer copy numbers compared with pUC-type high copy number plasmids, they are suitable for the expression of genes that may be toxic to host cells. The pSTV28 and pSTV29 vectors contain the pACYC184 origin of replication and can be co-transformed with pUC or pBR vectors.	2,999
pTV118N DNA	The pTV118N DNA, a phagemid vector, is constructed from a modified pUC118 plasmid. pTV118N DNA contains the sequence CCATGG, which includes the cleavage sequence for the restriction enzyme NcoI as well as the start codon (ATG) for lacZ. This enables target gene expression at the NcoI site, while protein expression is enabled by the vector's lac promoter. The pTV118N vector also contains a lacZ SD sequence. There are eight bases between the lacZ SD sequence and the initiation codon, allowing high level expression of target genes. Induction of single-stranded DNA by helper phage and its subsequent sequencing with RV-N primer enables accurate sequencing from the start codon site, ensuring an insert's correct translation frame.	3,162
pTWV228 DNA	The pTWV228 vector contains a pBR322 origin of replication, an ampicillin-resistance gene, an intergenic region (IG region) of the M13 phage DNA, and a beta-galactosidase gene containing the multiple cloning site of pUC118. This vector is low-copy number, which makes it useful during the expression of genes that present potential toxicity to their host.	4,039
pUC118 DNA	The pUC118 vectors can be used to prepare single-stranded DNA. pUC118 DNA was constructed by inserting the intergenic region (IG region) of the M13 phage DNA into a pUC18 plasmid. Co-transformation of pUC118 with the M13K07 helper phage induces the production of single-stranded DNA that is packaged into phage particles and released from bacterial cells. Using this system, large (up to 7 kb) and deletion-free single-stranded DNA can be stably obtained.	3,162

Storage

–20°C

Concentration

250–1,000 µg/ml (except as specifically listed below)

pTV118N: 0.5 µg/µl

pUC118 BAP-treated DNA: 0.1 µg/µl

pKF 18k-2 DNA: 0.5 µg/µl

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.