- Linkers and primers
- E. coli-yeast shuttle vectors
- Chloramphenicol-resistant pUC plasmids
- Kanamycin resistant pUC vectors
- pUC118/119 vectors
- pUC18 and pUC19 vectors
- DNA cloning vectors
- T-Vectors pMD20 and pMD19
- pPTR shuttle vectors
- M13mp18 virion DNA
- M13mp18 RF phage vector
- PhiX174 RF I DNA
- pBR322 DNA vector
- Thermus thermophilus HB8 genomic DNA
- Lambda DNA
- Plant transformation vectors
Pyrithiamine pPTR vectors
The pyrithiamine or pPTR vectors are E. coli-Aspergillus shuttle vectors and contain an ampicillin resistance gene (AmpR) as the selection marker for E. coli and a pyrithiamine-resistance gene (ptrA) as the selection marker for Aspergillus. Pyrithiamine is a pyridine analog and inhibitor of thiamine. Because the pPTR vector multiple cloning site (MCS) is located within the lacZ region, the presence or absence of target DNA insert can easily be verified using blue/white screening via bacterial plates containing IPTG and X-Gal.
- pPTR I DNA (4,782 bp): chromosomal integration vector for Aspergillus sp.
- pPTR II DNA (10,031 bp): autonomously replicating vector for Aspergillus sp.
- These vectors are supplied in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Selection of Aspergillus sp. transformants using pyrithiamine
Unique restriction sites
- pPTR I DNA: HindIII, PstI, SmaI, and KpnI
- pPTR II DNA: HindIII, SmaI, and KpnI
Do not use reagents containing thiamine during transformation, as traces of thiamine will decrease the bacterial selection efficiency of pyrithiamine media.
Kubodera, T., Yamashita, N. & Nishimura, A. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Biosci. Biotechnol. Biochem. 64, 1416–21 (2000).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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