PhiX174 RF I DNA
PhiX174 RF I DNA is a double-stranded circular DNA molecule composed of 5,386 base pairs. Restriction enzyme digest of phiX174 RF I DNA results in the generation of molecular weight size markers that can be used in nucleic acid gel analysis. PhiX174 double-stranded circular DNA is also frequently used in restriction enzyme quality control assays to assess endonuclease activity.
More Information
Source
Bacteriophage phiX174 am3
Purity
> 70% double-stranded covalently closed circular form I (RF I)
Storage
–20°C
GenBank
- Entry Name: phiX174, Accession No: NC_001422
Concentration
0.5 μg/μl
Product literature
Air, G. M. et al. Nucleotide sequence of the F protein coding region of bacteriophage phiX174 and the amino acid sequence of its product. J. Mol. Biol. 125, 247–54 (1978).
Sanger, F. et al. Nucleotide sequence of bacteriophage phi X174 DNA. Nature 265, 687–95 (1977).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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