DNA ligation kits, quick
DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 enable high-efficiency ligation of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these DNA ligation kits offer fast ligation in 30 minutes. Ligation reactions can be used directly in selected bacterial transformations with no need for DNA purification.
DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 enable high-efficiency ligation of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these DNA ligation kits offer fast ligation in 30 minutes. Ligation reactions can be used directly in selected bacterial transformations with no need for DNA purification.
DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 provide the fliexibility of formats, with the DNA Ligation Kit, Version 2.1 containing a convenient premix of enzyme and buffer that further minimizes pipetting steps and permits small final reaction volumes (for standard reactions). By using Solution II of the DNA Ligation Kit, Version 2.1, linker ligation may also be performed. Transformation efficiency can be further improved by the addition of Solution III (Transformation Enhancer) into circular ligation reactions prior to transformation. The DNA Ligation Kit, Version 1.0 can be used for DNA concatenation via the addition of Solution B alone.
More Information
Applications
- High-efficiency ligation in 3 minutes (30 minutes for most applications)
- Quick ligation for cloning in 3 minutes (30 minutes for some applications)
- DNA insertion into lambda phage vector (Ver. 1)
- Small-volume ligation (Ver. 2.1)
Version 1 components:
- Solution A: Reaction Buffer, 3 x 1 ml
- Solution B: Enzyme Solution, 2 x 187.5 µl
Version 2.1 components:
- Solution I: Enzyme Solution (contains ligase and reaction buffer), 3 x 250 µl
- Solution II: Concatenation Buffer, 1 x 750 µl
- Solution III: Transformation Enhancer, 1 x 200 µl
Product citation
Hayashi, K. et al., Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–7631 (1986).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab in the product table.
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