DNA ligation kits, quick

DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 enable high-efficiency ligation of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these DNA ligation kits offer fast ligation in 30 minutes. Ligation reactions can be used directly in selected bacterial transformations with no need for DNA purification.

DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 provide the fliexibility of formats, with the DNA Ligation Kit, Version 2.1 containing a convenient premix of enzyme and buffer that further minimizes pipetting steps and permits small final reaction volumes (for standard reactions). By using Solution II of the DNA Ligation Kit, Version 2.1, linker ligation may also be performed. Transformation efficiency can be further improved by the addition of Solution III (Transformation Enhancer) into circular ligation reactions prior to transformation. The DNA Ligation Kit, Version 1.0 can be used for DNA concatenation via the addition of Solution B alone.

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Cat. #	Product	Size	Price
6021	DNA Ligation Kit, Version 1	50 Rxns	$199.00
Takara's DNA Ligation Kits are simple, two-component systems that allow rapid performance of all cloning procedures requiring the covalent joining of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these kits allow ligation in 30 minutes with high transformation efficiency. For general ligation reactions, these kits allow good efficiency in only 3 minutes. The reactions can be used directly in some bacterial transformations without DNA purification. The kits have similar, simple procedures and give nearly equivalent transformation efficiencies. Version 1.0 can be used for DNA concatenation using Solution B alone. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back Fast ligation of a DNA fragment into a phage vector Fast ligation of a DNA fragment into a phage vector. Control insert DNA (plasmid vector) digested with EcoR I was ligated into dephosphorylated lambda gt11/EcoR I arms for 3 minutes at 25°C. Back Efficiency of Linker Ligation Efficiency of Linker Ligation. A pBgl II linker d[pCAGAATCTG] was ligated with dephosphorylated Hinc II pUC118 DNA. Ten nanograms of the ligation reaction was transformed into E. coli JM109 Competent Cells (1.3 x 10⁸ transformants/µg DNA), and the transformation efficiency was calculated from the yield of white colonies. Back DNA Ligation Kit recommended reaction conditions DNA Ligation Kit recommended reaction conditions. Back 6021: DNA Ligation Kit, Version 1
6022	DNA Ligation Kit, Version 2.1	50 Rxns	$199.00
Takara's DNA Ligation Kits are simple, two-component systems that allow rapid performance of all cloning procedures requiring the covalent joining of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these kits allow ligation in 30 minutes with high transformation efficiency. For general ligation reactions, these kits allow good efficiency in only 3 minutes. The reactions can be used directly in some bacterial transformations without DNA purification. The kits have similar, simple procedures and give nearly equivalent transformation efficiencies. However, Version 2.1 contains a convenient premix of enzyme and buffer that further minimizes pipetting steps and final reaction volume in standard reactions. Solution II of Version 2.1 is designed for linker addition ligations. Transformation efficiency can be improved by the addition of Solution III (Transformation Enhancer) into circular ligation reactions before transformation. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back 6022: DNA Ligation Kit, Version 2.1

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Applications

High-efficiency ligation in 3 minutes (30 minutes for most applications)
Quick ligation for cloning in 3 minutes (30 minutes for some applications)
DNA insertion into lambda phage vector (Ver. 1)
Small-volume ligation (Ver. 2.1)

Version 1 components:

Solution A: Reaction Buffer, 3 x 1 ml
Solution B: Enzyme Solution, 2 x 187.5 µl

Version 2.1 components:

Solution I: Enzyme Solution (contains ligase and reaction buffer), 3 x 250 µl
Solution II: Concatenation Buffer, 1 x 750 µl
Solution III: Transformation Enhancer, 1 x 200 µl

Product citation

Hayashi, K. et al., Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–7631 (1986).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab in the product table.

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