E. coli DNA Ligase
E. coli DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between double-stranded DNA fragments containing juxtaposed 5'-phosphate termini and 3'-hydroxyl termini in the presence of the NAD cofactor. Both E.coli DNA Ligase and T4 DNA Ligase are used in gene cloning experiments; however, E. coli DNA Ligase can only catalyze ligation of cohesive end-containing DNA fragments via standard reaction conditions.
- Gene cloning
- Phosphodiester bond formation
Recombinant E. coli
One unit is defined as the amount of enzyme that ligates >90% of 6 µg of HindIII-restricted DNA fragments in 30 min and 16°C (using a 20-µl reaction volume).
Panasenko, S. M., Alazard, R. J. & Lehman, I. R. A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lambda lysogen constructed in vitro. J. Biol. Chem. 253, 4590–2 (1978).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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