E. coli DNA Ligase
E. coli DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between double-stranded DNA fragments containing juxtaposed 5'-phosphate termini and 3'-hydroxyl termini in the presence of the NAD cofactor. Both E.coli DNA Ligase and T4 DNA Ligase are used in gene cloning experiments; however, E. coli DNA Ligase can only catalyze ligation of cohesive end-containing DNA fragments via standard reaction conditions.
- Gene cloning
- Phosphodiester bond formation
Panasenko, S. M., Alazard, R. J. & Lehman, I. R. A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lambda lysogen constructed in vitro. J. Biol. Chem. 253, 4590–2 (1978).
Additional product information
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