T4 RNA Ligase
T4 RNA Ligase is a ligation enzyme for joining single-stranded RNA or DNA fragments by catalyzing the formation of phosphodiester bonds between single-stranded RNA or DNA containing 5'-phosphate and 3'-hydroxyl termini. This enzyme requires ATP for activity, and its activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation, and lowest for DNA-DNA ligation. 3'-end labeling is often completed using 5'-[32P]pCp.
- Ligation of single-stranded RNA or DNA
- 3'-end labeling of single stranded RNA or DNA
E. coli B strain infected with T4 am N82
One unit is defined as the amount of enzyme that converts 1 pmol of [5'-32P]pCp into its acid-insoluble form in 10 minutes at 5°C, using oligo(A) as the substrate during 3' end labeling of RNA.
England, T. E., Bruce, A. G. & Uhlenbeck, O. C. Specific labeling of 3' termini of RNA with T4 RNA ligase. Methods Enzymol. 65, 65–74 (1980).
Romaniuk, P. J. & Uhlenbeck, O. C. Joining of RNA molecules with RNA ligase. Methods Enzymol. 100, 52–9 (1983).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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